BD Cell Viability Kit - TO evaporation?

23 July 2024 0 2K Report

Hello,

I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution.

I store the solutions according to manufacturer instructions and this happened with various kits (it is not a lot problem).

Does this happened to someone too?

Thank you!

Badges
Science topic

Similar topics
Filing

More Carolina Cassoni's questions See All

Best method for extracting RNA from morulae?

Best method and number of embryos for extracting RNA from pig morulae?

21 July 2024 4,397 1 View

Para Antropólogos/as: ¿Quisiera ser entrevistado?

Hola, Soy una estudiante de primer año de la carrera de Antropología en Chile, siendo parte del alumnado de la Universidad de Concepción. Se me ha asignado junto con mi grupo el trabajo de...

16 June 2024 2,715 4 View

How to measure de expansion of the cumulus?

Hello, I would like to inquire about how the expansion of the cumulus can be measured during or after IVM. Thank you.

16 May 2024 9,329 2 View

The best media for IVP in mice?

Hello community! What is for you the best combination of media for IVM, IVF and CIV in mice? In my experience we usually use media made in our laboratory but I have the opportunity to purchase...

02 May 2024 2,546 0 View

BLAST sequence in plus/minus and plus/plus in reverse sequence when CRISPR is performed. Are they mutants?

We are performing CRISPR on a gene from a strain of Aspergillus niger and then sequencing the results by Sanger. When we compare the gene sequence with the resulting sequence we observe in the...

25 April 2024 6,159 0 View

Determination of Tocopherols and phytosterols by GC-MS without derivatization?

Hello, I need to determine tocopheroels and phytosterols by GC-MS but I do not have the reagents to derivatize (TMS, TMCS, BSTFA, etc.) that most of the consulted literature generally uses. I...

22 April 2024 8,177 6 View

Where can I get Biological Materials Science Book (Myers and Chen, 2014)?

I know it may be controversial, and I favour crediting the authors. However, knowledge should also be accessible to student researchers. My doctoral colleague and I would like to access the book,...

12 March 2024 1,333 4 View

How to calculate the sample size calculation for an in vitro acrylic resin study?

I need to make my sample size calculation without a pilot study, my n=10, but how can I prove that I have this number?

02 January 2024 9,533 1 View

How can I get calcium alginate at home with sodium alginate and calcium chloride?

Dear Researchers, I am looking for ratios of sodium alginate (powder) and calcium chloride (flakes/ granulate) and processes to create calcium alginate in FabLab facilities. PS: I am doing my...

12 December 2023 6,434 5 View

Detection limit for the viral titers assay. Can someone help me??

The reviewer requested that the authors indicate the detection limit for the viral titers assay. However, the assay was performed using PFU, and I am unsure how to determine the assay's detection...

20 November 2023 9,080 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,822 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,104 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,726 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,365 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,911 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View