Is there a dataset available on actual individual person usage of OS? If not what is the best way to create it?

I want to create a N.N which can learn person OS usage based on their use of application and data related to specific application's background time, usage frequency, usage time of the application...

07 December 2020 1,252 4 View

What is causing the delay in expression of EGFP in HEK293T transient transfection with pAAV plasmid?

I have an AAV2 backbone and I am trying to create viruses for my further experiments. I am making an AAV CMV EGFP virus as a negative control and it is giving me a problem. The EGFP expression in...

15 January 2020 4,057 1 View

How can I interpret the negative value of regression coefficient in logistic regression??

I am running a logistic regression by using dichotomous dependent variable and five independent variable. I found one of the independent variable is getting -ve regression coefficient. 1) What...

07 November 2017 4,464 9 View

How Gray level co-occurrence matrix or GLCM calculated for an image is implemented on that image for texture feature extraction?

Information such as contrast, entropy, dissimilarity index and variance are computed to know the properties of any image, but how these values are used to for further classification or feature...

12 October 2017 4,978 3 View

Entering the field of Data Science as a developer

Being a python programmer, I have decided to get in the field of data science and later on AI and Machine Learning. I have already brushed up the knowledge of statistics and other python libraries...

23 August 2017 5,999 13 View

What assay I should perform for insulin receptor activation?

I want to check anti diabetic effect by insulin receptor activation. Which assay i could perform to check insulin receptor activation.

26 April 2017 1,835 2 View

Is atmospheric correction mandatory for chlorophyll estimation from Landsat images and which is best method of atmospheric correction?

I am trying to estimate chlorophyll-a by using Landsat/ MODIS image. So I want to know atmospheric correction for the above analysis is required or not? There is so much technique for atmospheric...

08 March 2016 9,488 2 View

How can I calculate the load of a virtual machine?

In a virtual machine there is a migration. Let's suppose i want to select a virtual machine, based on it's current load, and make a decision based on the current load to migrate virtual machine....

06 September 2013 9,969 18 View

How to measure OFDM PAPR in Matlab Simulink?

01 January 1970 7,579 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View