We have NIT cell lines, what are the assay should I use for checking insulin receptor activation ?
Dear Harshit, any common signal downstream if the IR, like Akt pSer473 should do it.
Plant tissue culture: ammonium sulfate instead of ammonium nitrate? what concentration? how to prepare stock 1 solution for MS media using ammonium sulfate?
07 May 2023 4,177 3 View
I have been trying to purify Amyloid Beta peptide using ion exchange chromatography. In the paper I am referring to, they have used DEAE Cellulose in their protocol. I have been using Q-Sepharose...
15 January 2023 2,501 0 View
Dear all, I have been doing trials for accessing corrosion properties of steel rebar in chloride environment using Potentiostat, wherein my working electrode is steel rebar, reference electrode is...
15 November 2022 8,797 0 View
is there any traditional method to check T cell levels or can we isolate T-cells to check Th17 cell levels using specific markers? I am a little stuck here. Just to clarify I don't want to use...
20 September 2022 1,266 1 View
I get callus from chickpea genotype but i want to know which types of media and hormones concentration required for generation of shoot from callus
30 August 2022 637 0 View
I need a rapid protocol for the detection of any spectral signature of nanoparticles inside plant tissues without necessarily sectioning the tissue. Is it possible?
28 August 2022 2,370 0 View
What is best protocol for estimation of proline, MDA content Catalase and SOD for plant leaves ?
02 June 2022 2,229 2 View
what is best method of surface sterilization of explant in tissue culture ? and how to minimize fungal contamination in tissue culture ? Can I use Fungicide for surface sterilization of explant...
02 May 2022 8,709 4 View
I'm conducting research to understand the design management practices in early stage start-ups. I 'm taking a action research and case study method as my approach. Would like to connect with...
14 February 2022 7,613 0 View
I am an M.Tech structural engineering student working on the project ' Numerical analysis of Kath-kuni architecture ( a common masonry typology ) in Himachal Pradesh region of India subjected to...
11 January 2022 1,154 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
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05 August 2024 4,407 0 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View