Plant tissue culture: ammonium sulfate instead of ammonium nitrate? what concentration? how to prepare stock 1 solution for MS media using ammonium sulfate?
Dear Sagar Jadav if you replace NH4NO3 with (NH4)2SO4, then, it's not the MS formulation but a new one. Besides, you must to consider that the introduction of new salts will affect the whole composition, and balance of formulation. Therefore, likely you would need to perform further adjustments in the new formulation.
Ammonium sulfate can be used as a source of nitrogen in plant tissue culture media instead of ammonium nitrate. The concentration of ammonium sulfate to be used in the tissue culture medium depends on the specific plant species and the growth stage of the plant tissue being cultured.
In general, the concentration of ammonium sulfate in tissue culture media ranges from 500 mg/L to 1000 mg/L. However, it is important to note that ammonium sulfate is less soluble than ammonium nitrate, which can make it more difficult to dissolve and prepare the media. It is recommended to adjust the pH of the media after adding the ammonium sulfate to ensure that the pH is appropriate for the specific plant species being cultured.
It is also important to consider the potential toxic effects of ammonium ions on plant tissue. High concentrations of ammonium ions can be toxic to plant cells and tissues, and can inhibit growth and development. Therefore, it is important to optimize the concentration of ammonium sulfate in the tissue culture media to avoid toxicity and to promote healthy growth and development of the plant tissue.
Dear Sagar Jadav, nitrate, and ammonium are usually employed in the plant tissue media. Nitrate is an important source of elemental nitrogen for most of the tissue culture media. The combination of nitrate and ammonium in tissue culture media is more conductive towards growth than either (NO3-) or (NH4+). As Ricardo Julian Licea-Moreno mentioned earlier that you cant replace ammonium nitrate with the ammonium sulphate. If you replace the ammonium nitrate with the ammonium sulphate, the Murashige and Skoog (MS) formulation will change. Consequently, you need to perform different experimental setup for further adjustment in the new formulation.
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