I get callus from chickpea genotype but i want to know which types of media and hormones concentration required for generation of shoot from callus
How to enhanced h-index in Research Gate?
04 August 2024 3,368 4 View
why uv is done and why photocatalytic measurement is done although both use uv radiation
09 June 2024 8,750 1 View
it would be helpful if you provide the reference to your answer
11 May 2024 5,989 4 View
I just completed my M.Sc Physics while researching on ferrites in Photocatalytic property. Now i want to do My Phd does anyone suggest me any topic or any good university for doing my reserch
19 April 2024 3,772 2 View
Hello, RG Members, I have designed the COBRA primers using the UCSC genome browser for the promoter regions of genes of my intreset. are these primers reliable? I have tried the other softwares...
17 April 2024 7,882 0 View
I'm working on sugars present in honey sample.I tried paper and TLC with different mobile phases like Butanol:Glacial Acetic acid:Water and Butanol:Pyridine:Water but I'm not getting expected...
05 March 2024 9,609 1 View
I'm working on utilization of wastes for biogas production.I streak a loopful cowdung slurry collected from biogas plant on sodium thioglycolate media.I got some colonies on the media.I need to...
28 February 2024 1,877 4 View
Can anyone knows about how to find the restriction sites using rs IDs. Are there any tools to find?
21 February 2024 2,794 0 View
I'm trying to seprate amino acids using silica gel.When I spray ninhydrin solution on silica gel it get disturbed.Can anyone tell me how to observe separated amino acid on silica gel plate?
06 February 2024 8,366 2 View
Hello everyone, This is my first question and very curious to learn why my GST fused protein shows band in the cell Lysate flow through, lysis buffer, wash buffer, but absolutely no bands on the...
25 January 2024 7,470 3 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
Hai, Iam doing experiment about novel compound to induce thermogenesis on 3t3-L1 cells. The beta actin has been adjusted, but when i did western blot and imaging with image J, the result it is...
14 July 2024 3,354 1 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...
30 June 2024 6,502 5 View
Hi everyone, I am currently trouble shooting my restriction digestion, so far it hasnt given me clear bands when I ran gel. I've been adding all reagents minus template, to make a master mix...
24 June 2024 1,171 3 View
What food do intestinal bacteria like most?ChatGPTIntestinal bacteria love fibrous foods that are prebiotic. These foods include vegetables (such as broccoli, cabbage, and brussels sprouts),...
22 June 2024 4,567 0 View
We coat the plate in poly-D-lysine (50 ug/mL) followed by fibronectin (50 ug/mL) before plating the cardiomyocytes. After isolating and plating cardiomyocytes from 2-4 day old pups, we fed the...
20 June 2024 393 0 View
can any one provide shooting method in matlab for 9th order differential equations with 9 boundry condition(Velocity, Temperature and concentration)
14 June 2024 7,928 2 View
I know that all animals and insects produces hormones. Do plants produce hormones that can have an equivalent effect on an animal like animal hormones?
13 June 2024 3,860 1 View