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Dear Experts, I'm trying to separate a Fluorescein-like Xanthene dye (only phenolic and carboxylic charged groups) from byproducts with amino groups. I assumed that a strong acidic cation...
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What would you recommend? It should be cheap and of constant / defined composition, comparable to defatted milk powder (NFDM). Thanks!
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Dear experts, I am planning a clean-up step in a protein purification process where I actually thought of using Sephacryl S200. In the stock room, I found some Sephadex G200 and thought, I'd give...
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Dear Colleagues, peptide synthesis companies offer different purities at different prices.What actually defines the purity? Several things come into my mind: - sequence errors (i.e. usually...
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Dear colleagues, I have designed a construct for eukaryotic expression. Before I submit it for synthesis, I would like to find out about the possible implications of 3 (unintended) features that...
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Does anyone have a good resource to share on describing the colorimetric signal in sandwich ELISA mathematically as a function of antibody- and antigen concentration and other variables? In order...
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