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Questions related from Wolfgang Schechinger
I am observing that some people (or bots?) are posting more and more answers which obviously are generated by AI chatbots. As these answers IMHO reflect the stupidity of the Internet (that what...
24 July 2023 3,757 2 View
Dear Experts, I'm trying to separate a Fluorescein-like Xanthene dye (only phenolic and carboxylic charged groups) from byproducts with amino groups. I assumed that a strong acidic cation...
23 September 2021 3,734 0 View
What would you recommend? It should be cheap and of constant / defined composition, comparable to defatted milk powder (NFDM). Thanks!
11 January 2018 7,699 6 View
Dear experts, I am planning a clean-up step in a protein purification process where I actually thought of using Sephacryl S200. In the stock room, I found some Sephadex G200 and thought, I'd give...
04 December 2015 9,937 5 View
Dear Colleagues, Any suggestions for alternatives to cyanoborohydride (in respect of less toxic, cheaper, suitable for aqueous settings)? It's about the reductive amination of a polysaccharide...
12 March 2015 3,762 9 View
Dear Colleagues, peptide synthesis companies offer different purities at different prices.What actually defines the purity? Several things come into my mind: - sequence errors (i.e. usually...
03 February 2015 4,954 6 View
Dear colleagues, I have designed a construct for eukaryotic expression. Before I submit it for synthesis, I would like to find out about the possible implications of 3 (unintended) features that...
25 August 2014 5,640 1 View
This question is about reacting aldehydes and ketones with hydrazine. I would like to prepare an asymmetric azine: I'm thinking about adding aldehyde 1 (eg salicylic aldehyde) to an excess of...
14 March 2013 5,551 6 View
I am trying to purify an antibody by affinity chromatography on the immobilized antigen, a 1300Da synthetic peptide, bound via its N-terminus on NHS activated sepharose (the only amino group in...
19 February 2013 7,477 6 View
Does anyone have a good resource to share on describing the colorimetric signal in sandwich ELISA mathematically as a function of antibody- and antigen concentration and other variables? In order...
18 October 2012 6,786 6 View
