I need a rapid protocol for the detection of any spectral signature of nanoparticles inside plant tissues without necessarily sectioning the tissue. Is it possible?
I want to create a N.N which can learn person OS usage based on their use of application and data related to specific application's background time, usage frequency, usage time of the application...
07 December 2020 1,252 4 View
I have an AAV2 backbone and I am trying to create viruses for my further experiments. I am making an AAV CMV EGFP virus as a negative control and it is giving me a problem. The EGFP expression in...
15 January 2020 4,057 1 View
Please help me in understanding the hairy root initiation condition. some paper suggest that after co cultivation in dark the explant without further bacterial infection outside is maintained on...
03 September 2019 4,639 4 View
Information such as contrast, entropy, dissimilarity index and variance are computed to know the properties of any image, but how these values are used to for further classification or feature...
12 October 2017 4,978 3 View
Being a python programmer, I have decided to get in the field of data science and later on AI and Machine Learning. I have already brushed up the knowledge of statistics and other python libraries...
23 August 2017 5,999 13 View
We have NIT cell lines, what are the assay should I use for checking insulin receptor activation ?
10 May 2017 9,836 1 View
I want to check anti diabetic effect by insulin receptor activation. Which assay i could perform to check insulin receptor activation.
26 April 2017 1,835 2 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...
01 March 2021 3,610 1 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...
01 March 2021 9,511 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...
24 February 2021 9,210 1 View