Does anyone have this YT-1 strain for research purpose? If anyone has then please reply to this post.
I want to perform PCR of high risk genotypes, using specific as well as consensus primers.
05 June 2016 8,055 3 View
I want to use e6 primer for detection of HPV 16 in the cervical swab. I would like to know the PCR program to run this primer amplification. I
05 June 2016 8,537 2 View
While performing HLAB27 typing through allele specific PCR, why we use HgH AS INTERNAL CONTROL?
05 June 2016 1,589 2 View
I would like to know if any institution in Kathmandu has facility for lyophilizing the bacteria for research purpose?
05 June 2016 7,134 2 View
I am trying to perform sequencing of different antibiotic genes carrying plasmid. I have amplified the respective genes separately with pcr and now want to sequence them. Can anyone suggest me...
05 June 2016 5,233 4 View
I would like to know which international journal with real impact factors publish microbiology research papers without article processing charge. I am asking about journals other that plos, BMC
04 May 2016 8,912 0 View
what is the broad/universal primer for amplification of HPV. I have searched and found universal primer MY09 and MY11 and SPF10 using the L1 . But i couldnot finf the primer sequence. I would...
03 April 2016 6,161 2 View
While ordering the MY09 AND MY 11 primers, the primer sequence as per literature is: 5’CGTCCMARRGGAWACTGATC3’ and 5’GCMCAGGGWCATAAYAATGG3. what does M,R,W, represents and how should i order the...
03 April 2016 7,888 3 View
Being a researcher and associated with small laboratory of NEPAL , how can i apply for small grants and where to apply for a research Grant. I would like to know the procedures involved.
02 March 2016 10,029 0 View
What are some of the molecular miRNA researches and projects that can be done in small laboratories as a project work.
02 March 2016 7,698 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View