Can anyone provide me hpv 16/18 positive controls for molecular diagnosis by PCR?

I want to perform PCR of high risk genotypes, using specific as well as consensus primers.

05 June 2016 7,965 3 View

Why we use HgH internal control primer in HLAB27 typing?

While performing HLAB27 typing through allele specific PCR, why we use HgH AS INTERNAL CONTROL?

05 June 2016 1,491 2 View

PCR program for HPV detection using e6 primer?

I want to use e6 primer for detection of HPV 16 in the cervical swab. I would like to know the PCR program to run this primer amplification. I

05 June 2016 8,439 2 View

Where can i get lyophilizer (service) for bacteria in Kathmandu ?

I would like to know if any institution in Kathmandu has facility for lyophilizing the bacteria for research purpose?

05 June 2016 7,049 2 View

Which primer to use for sequencing plasmid?

I am trying to perform sequencing of different antibiotic genes carrying plasmid. I have amplified the respective genes separately with pcr and now want to sequence them. Can anyone suggest me...

05 June 2016 5,141 4 View

Which are the Impact journals with no article processing charge?

04 May 2016 8,820 0 View

HPV universal primer sequence?

03 April 2016 6,084 2 View

Universal primer of HPV, MY 09 AND MY 11?

03 April 2016 7,797 3 View

Applying for a small research grant?

Being a researcher and associated with small laboratory of NEPAL , how can i apply for small grants and where to apply for a research Grant. I would like to know the procedures involved.

02 March 2016 9,936 0 View

MiRNA cancer researches in small laboratories?

What are some of the molecular miRNA researches and projects that can be done in small laboratories as a project work.

02 March 2016 7,621 0 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View