what is the broad/universal primer for amplification of HPV. I have searched and found universal primer MY09 and MY11 and SPF10 using the L1 . But i couldnot finf the primer sequence. I would like to know the full sequence of these primers.
Hi Hriush,
MY09/MY11 is a very common degenerate primer set for HPV L1 gene amplification and detection. Please find some attached articles.
S Datta
U Just go to this Accession number on NCBI web......
Accession number: AY262282
I want to perform PCR of high risk genotypes, using specific as well as consensus primers.
05 June 2016 8,055 3 View
I want to use e6 primer for detection of HPV 16 in the cervical swab. I would like to know the PCR program to run this primer amplification. I
05 June 2016 8,537 2 View
While performing HLAB27 typing through allele specific PCR, why we use HgH AS INTERNAL CONTROL?
05 June 2016 1,589 2 View
I would like to know if any institution in Kathmandu has facility for lyophilizing the bacteria for research purpose?
05 June 2016 7,134 2 View
I am trying to perform sequencing of different antibiotic genes carrying plasmid. I have amplified the respective genes separately with pcr and now want to sequence them. Can anyone suggest me...
05 June 2016 5,233 4 View
I would like to know which international journal with real impact factors publish microbiology research papers without article processing charge. I am asking about journals other that plos, BMC
04 May 2016 8,912 0 View
While ordering the MY09 AND MY 11 primers, the primer sequence as per literature is: 5’CGTCCMARRGGAWACTGATC3’ and 5’GCMCAGGGWCATAAYAATGG3. what does M,R,W, represents and how should i order the...
03 April 2016 7,888 3 View
Being a researcher and associated with small laboratory of NEPAL , how can i apply for small grants and where to apply for a research Grant. I would like to know the procedures involved.
02 March 2016 10,029 0 View
What are some of the molecular miRNA researches and projects that can be done in small laboratories as a project work.
02 March 2016 7,698 0 View
I would like to know what are some bacteria that are present in the soil samples of high altitude land approx 3000m Above sea level. While isolating the bacteria from soil samples of this site...
02 March 2016 4,345 5 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View