Can anyone provide me hpv 16/18 positive controls for molecular diagnosis by PCR?

I want to perform PCR of high risk genotypes, using specific as well as consensus primers.

05 June 2016 7,965 3 View

Why we use HgH internal control primer in HLAB27 typing?

While performing HLAB27 typing through allele specific PCR, why we use HgH AS INTERNAL CONTROL?

05 June 2016 1,491 2 View

Where can i get lyophilizer (service) for bacteria in Kathmandu ?

I would like to know if any institution in Kathmandu has facility for lyophilizing the bacteria for research purpose?

05 June 2016 7,049 2 View

Which primer to use for sequencing plasmid?

I am trying to perform sequencing of different antibiotic genes carrying plasmid. I have amplified the respective genes separately with pcr and now want to sequence them. Can anyone suggest me...

05 June 2016 5,141 4 View

Which are the Impact journals with no article processing charge?

04 May 2016 8,820 0 View

HPV universal primer sequence?

03 April 2016 6,084 2 View

Universal primer of HPV, MY 09 AND MY 11?

03 April 2016 7,797 3 View

Applying for a small research grant?

Being a researcher and associated with small laboratory of NEPAL , how can i apply for small grants and where to apply for a research Grant. I would like to know the procedures involved.

02 March 2016 9,936 0 View

MiRNA cancer researches in small laboratories?

What are some of the molecular miRNA researches and projects that can be done in small laboratories as a project work.

02 March 2016 7,621 0 View

Bacteria from high altitude?

I would like to know what are some bacteria that are present in the soil samples of high altitude land approx 3000m Above sea level. While isolating the bacteria from soil samples of this site...

02 March 2016 4,253 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View