Can anyone provide me hpv 16/18 positive controls for molecular diagnosis by PCR?

I want to perform PCR of high risk genotypes, using specific as well as consensus primers.

05 June 2016 7,965 3 View

Why we use HgH internal control primer in HLAB27 typing?

While performing HLAB27 typing through allele specific PCR, why we use HgH AS INTERNAL CONTROL?

05 June 2016 1,491 2 View

PCR program for HPV detection using e6 primer?

I want to use e6 primer for detection of HPV 16 in the cervical swab. I would like to know the PCR program to run this primer amplification. I

05 June 2016 8,439 2 View

Which primer to use for sequencing plasmid?

I am trying to perform sequencing of different antibiotic genes carrying plasmid. I have amplified the respective genes separately with pcr and now want to sequence them. Can anyone suggest me...

05 June 2016 5,141 4 View

Which are the Impact journals with no article processing charge?

04 May 2016 8,820 0 View

HPV universal primer sequence?

03 April 2016 6,084 2 View

Universal primer of HPV, MY 09 AND MY 11?

03 April 2016 7,797 3 View

Applying for a small research grant?

Being a researcher and associated with small laboratory of NEPAL , how can i apply for small grants and where to apply for a research Grant. I would like to know the procedures involved.

02 March 2016 9,936 0 View

MiRNA cancer researches in small laboratories?

What are some of the molecular miRNA researches and projects that can be done in small laboratories as a project work.

02 March 2016 7,621 0 View

Bacteria from high altitude?

I would like to know what are some bacteria that are present in the soil samples of high altitude land approx 3000m Above sea level. While isolating the bacteria from soil samples of this site...

02 March 2016 4,253 5 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 1,808 1 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View