Can anyone provide me hpv 16/18 positive controls for molecular diagnosis by PCR?

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PCR program for HPV detection using e6 primer?

I want to use e6 primer for detection of HPV 16 in the cervical swab. I would like to know the PCR program to run this primer amplification. I

05 June 2016 8,537 2 View

Why we use HgH internal control primer in HLAB27 typing?

While performing HLAB27 typing through allele specific PCR, why we use HgH AS INTERNAL CONTROL?

05 June 2016 1,589 2 View

Where can i get lyophilizer (service) for bacteria in Kathmandu ?

I would like to know if any institution in Kathmandu has facility for lyophilizing the bacteria for research purpose?

05 June 2016 7,134 2 View

Which primer to use for sequencing plasmid?

I am trying to perform sequencing of different antibiotic genes carrying plasmid. I have amplified the respective genes separately with pcr and now want to sequence them. Can anyone suggest me...

05 June 2016 5,233 4 View

Which are the Impact journals with no article processing charge?

I would like to know which international journal with real impact factors publish microbiology research papers without article processing charge. I am asking about journals other that plos, BMC

04 May 2016 8,912 0 View

HPV universal primer sequence?

what is the broad/universal primer for amplification of HPV. I have searched and found universal primer MY09 and MY11 and SPF10 using the L1 . But i couldnot finf the primer sequence. I would...

03 April 2016 6,161 2 View

Universal primer of HPV, MY 09 AND MY 11?

While ordering the MY09 AND MY 11 primers, the primer sequence as per literature is: 5’CGTCCMARRGGAWACTGATC3’ and 5’GCMCAGGGWCATAAYAATGG3. what does M,R,W, represents and how should i order the...

03 April 2016 7,888 3 View

Applying for a small research grant?

Being a researcher and associated with small laboratory of NEPAL , how can i apply for small grants and where to apply for a research Grant. I would like to know the procedures involved.

02 March 2016 10,029 0 View

MiRNA cancer researches in small laboratories?

What are some of the molecular miRNA researches and projects that can be done in small laboratories as a project work.

02 March 2016 7,698 0 View

Bacteria from high altitude?

I would like to know what are some bacteria that are present in the soil samples of high altitude land approx 3000m Above sea level. While isolating the bacteria from soil samples of this site...

02 March 2016 4,345 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Putra Dwija

I did HPV PCR using consensus primer (my9/11) and specific type, first I just try to pcr from the specimen, and when positive I use that specimen as a positive control...

Hriush Adhikari

Mam Can you please provide the pcr condition ( program) you used for this amplification?

Stefan Butureanu

The best is a Cobas machine made by Roche