Solubility of calcium ionophore II (ETH-129)?

04 September 2019 1 6K Report

Hello all - I am trying to add the calcium ionophore ETH-129 from Sigma ( https://www.sigmaaldrich.com/catalog/product/sial/21193?lang=en®ion=US ) to phospholipid bilayer/vesicles. The only methods I've seen in the literature have dissolved ETH-129 in ethanol, but I'm worried that placing these bilayers/vesicles into direct contact with ethanol may compromise its barrier properties, as the individual lipids are soluble in organic solvents. Is this a valid concern?

It would be nice to add the ETH-129 as the vesicles are formed, but the lipids are dissolved in chloroform. Is ETH-129 soluble in apolar organic solvents? Or could the ionophore hold its tertiary structure after vacuum evaporation?

Noemi Tejera

Hi Emily

Is not DMSO a possibility? I believe that up to 1% should be safe to use

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