What are these substances forming in my cell culture?

More Emily Burdick's questions See All

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Can a random-intercept cross lagged panel model (RI-CLPM) be indentified with just two measurement points?

I am currently planning out my masters' thesis design and will be working with a large data set with variables that have been meseasured at two time points. Intially I thought of just using a...

21 January 2021 9,384 4 View

Why are species accumulation curves shorter in R than Primer?

I have previously used Primer to output species accumulation curve results (including Chao, Jacknife and Bootstrap estimators), and from memory if I had 40 study sites, there would be 40 numbers...

23 December 2020 8,514 2 View

How to calculate sample size for 3 way Mixed Between Within Subjects ANOVA?

Hi all, I have been trying to figure out a way to calculate a priori sample size for a mixed anova. I have a study that uses 2 x 7 x 2. The last IV is within subjects. I appreciate any help....

23 September 2020 3,635 3 View

What opinions do you have on plate readers you've used?

Hello everyone, Our lab is currently looking to purchase a plate reader and I was interested in seeing what opinions people have about some that they have used. Some of the other labs on...

07 September 2020 2,736 3 View

How to do metaphase spread IF staining?

Pls help! I would like to do IF staining with 2 antibodies on a metaphase spread. But I dont have a cytospin! How to do it without cytospin? And im using methanol:acetic acid fixation.

16 August 2020 488 1 View

Qualitative Interview Translations- Best transcription services for Spanish audio to English text?

For an upcoming research project I will have audio files of interviews conducted in Spanish. I am looking for a company that can transcribe and translate these interviews - resulting in English...

28 June 2020 769 2 View

Is it possible to remove pollen from museum bee specimens?

I have recently extracted genomic DNA from some museum bees for sequencing, and from first look there appears to be some pollen DNA in the extract. I've had a Google but can't find anything...

25 May 2020 8,423 2 View

What is the best way to probe for Pyk2 activation?

I have been trying to look at Pyk2 phosphorylation in my cells (human monocyte derived macrophages) for about a year via Western Blot, and am having a massive amount of difficulty doing so. I have...

16 April 2020 6,662 0 View

What effect size calculation to use for meta-analysis?

I am conducting a meta-analysis for the association between TNBC and BRCA1 and BRCA2. Can anybody help me as to what 2x2 frequency table to use- standardised mean difference, correlation...

05 March 2020 4,992 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Vatamanu Sergiu-Marian

It might be protein precipitating from serum, is it a free FBS medium? At what temp do you store the drug RITA?

Jp Wu

If a Raman microscope is available, it can identify the particles precipitated from your sample. They could probably be the crystals of your drug substance, in its input or different polymorphic/salt forms.