I am conducting a cell viability assay on a large sample of adherent cells unable to be distributed into a traditional sized 96 well plate. How would I analyze this larger sample? Would an ATP Glo or MTT assay be better? I will be dosing the cells with a known carcinogen followed by a treated media and then measuring viability.
Hello Emily Burdick
If I have understood your question correctly, are you looking for something like a 384-well microtiter plate or 1536-well microtiter plate format instead of the usual 96-well plate format to accommodate a large sample size?
If I am right, then for the cell viability assay, you should select an assay that is simple, rapid, sensitive, and cost effective. Instead of ATP Glo or MTT assay, you should go for Alamar Blue assay, which is based on a dye called resazurin, a nonfluorescent and blue dye which upon reduction (by the natural reducing power of living cells) to resorufin becomes pink and highly fluorescent.
It does not require washing steps which makes it easily amenable to miniaturization and automation. This agent is widely used to measure cell viability by prompt fluorescence (excitation: 531 nm, emission: 595 nm). The fluorescence can be easily detected using a microtiter plate reader.
For more information you may want to refer to the article attached below.
Article A high density assay format for the detection of novel cytot...
Hope this helps!
Best.
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