By using fractional distillation, you can effectively separate the isopropanol from the water and recover a high purity isopropanol fraction for reuse. The key is to carefully monitor the distillation temperature to collect the isopropanol fraction before the water comes over.

to recover and reuse isopropanol from a yeast extraction protocol using NaOH, the key steps would be:

1-Neutralize the NaOH if present in high concentration, such as by adding an acid like hydrochloric acid to form a salt that can be filtered out.

2- Break the azeotropic mixture of isopropanol and water, either by adding a salt or using a desiccant like molecular sieves to selectively absorb the water.

3- Distill off the purified isopropanol, potentially using fractional distillation to improve the separation, so it can be reused in future extractions. While complete recovery may not be possible, these techniques can help maximize the reuse of the isopropanol solvent.