Should I change the buffer of my protein solution before doing SDS PAGE?

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Omar Gonzalez-Ortega

There is no need to, pH will be established by the sample (Laemmli) buffer.

Himanshu Antil

There is no need to change the buffer.

Shintaro Iwashita

Dear Torsha,

As you imagine, protein mobility on SDS-PAGE is very relative depending on the context of the sample. Therefore, care must be taken to adjust the content of the samples to be compared. If it is difficult to adjust, mix and use as a control (Please see Figure 7 at https://doi.org/10.1042/BSR20150111).