Torsha Ghosh

8 Questions 6 Answers 0 Followers

Questions related from Torsha Ghosh

How can i distinguish biofilm or some contamination?

I am first time doing bacteria experiment without any prior practice. I am trying to make bifidobacterium biofilm. According to references i am incubating them in different concentration of bile...

24 December 2023 7,618 5 View

How to retrive bacteria frim a brith medium that contains blood?

This is my first time culturing and working with bacteria. I am culturing Bifidobacteria in the anaerobic gas chamber. According to the references I am using BL broth media supplemented with 5%...

23 January 2023 6,415 1 View

How to store a bacteria with biofilm for future use in in vivo experiment?

This is my first time doing experiments with bacteria. I use probiotic to alleviate disease state. I use live bacteria for oral gavage. I am comaring probiotic and probiotic with biofilm. For...

24 July 2022 7,837 6 View

Should I change the buffer of my protein solution before doing SDS PAGE?

I have PEGylated antibody in 6.5 pH phosphate buffer. I want to show change in molecular weight using SDS PAGE. While preparing the sample should I change the buffer of my sample to TRIS-HCl pH...

09 July 2021 1,198 3 View

Protein sample in 10 pH borate buffer can be used for SDS PAGE directly?

I have PEG conjugated antibody in pH 10 borate buffer. I have to run SDS PAGE to determine MW. In this case prior running the gel do I need to do buffer change of the sample? Like borate buffer to...

17 May 2021 5,553 2 View

How do I produce inflammatory type of CAF from fibroblast or stellate cells?

I want to differentiate fibroblast to heterogeneous CAFs in vitro. Direct or indirect incubation with cancer cell is one optio. If I incubate fibroblast with specific cytokines, will I get...

27 January 2021 6,950 1 View

I am culturing both macrophage and cancer cell together as spheroid. I want to use RPMI and DMEM media at 1:1 ratio. Is there any disadv of this?

I am culturing macrophages and tumor spheroid to study cross talk. now as preferred media for macrophage is RPMI and cancer cell is DMEM i am confused what to use. I want to mix them in 1:1 ratio....

19 February 2019 5,827 0 View

What is the usual ABTS assay for transition metal complex with antibiotics? My product is soluble in methanol.

There are different protocol described. So I am confused which one to follow for ABTS assay.

12 August 2017 3,146 1 View