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I am first time doing bacteria experiment without any prior practice. I am trying to make bifidobacterium biofilm. According to references i am incubating them in different concentration of bile...
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This is my first time culturing and working with bacteria. I am culturing Bifidobacteria in the anaerobic gas chamber. According to the references I am using BL broth media supplemented with 5%...
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This is my first time doing experiments with bacteria. I use probiotic to alleviate disease state. I use live bacteria for oral gavage. I am comaring probiotic and probiotic with biofilm. For...
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I have PEGylated antibody in 6.5 pH phosphate buffer. I want to show change in molecular weight using SDS PAGE. While preparing the sample should I change the buffer of my sample to TRIS-HCl pH...
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I have PEG conjugated antibody in pH 10 borate buffer. I have to run SDS PAGE to determine MW. In this case prior running the gel do I need to do buffer change of the sample? Like borate buffer to...
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I want to differentiate fibroblast to heterogeneous CAFs in vitro. Direct or indirect incubation with cancer cell is one optio. If I incubate fibroblast with specific cytokines, will I get...
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I am culturing macrophages and tumor spheroid to study cross talk. now as preferred media for macrophage is RPMI and cancer cell is DMEM i am confused what to use. I want to mix them in 1:1 ratio....
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