Hello all. In lab we grow cells to secret mHTT in media that we then collect and subject to centrigufation at 100k G for 2 hours to concentrate the protein and other secreted components of the media.
If I wanted to run this through a western, what would I resuspend the resulting pellet/mass in? Typically we just do PBS, but should I use the lysis buffer that's typical of western blots?
Thanks.
Engelbert Buxbaum
For WB analysis, you solubilise the protein in Laemli's sample buffer. Make sure that the final concentration of the buffer is 1x, using dd water as diluent.
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