How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

Would bisulfide sequencing result be affected if the tissue is fixed by perfusion?

I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...

09 February 2021 9,012 3 View

Alpha and dense granules in platelets not visible in Transmission Electron Microscopy?

I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...

04 February 2021 5,925 9 View

Why E1g mode (Molybdenum disulfide) is undetectable in Raman spectroscopy?

I synthesized 1T/2H hybrid phase MoS2 on a graphite foil surface by hydrothermal method, and then analyzed it by Raman spectroscopy. As a result, three new peaks correspond to the 1T phase were...

04 February 2021 6,580 2 View

(abaqus) Questions about the uniform stress condition of spherical particles ??

I want to simulate a uniform stress condition in the vertical direction on the surface of a spherical particle. Since it is very difficult to describe the boundary conditions of a complete...

31 January 2021 2,726 1 View

How do they fill/replace metal hydrides in vessels?

Surely they will want to avoid contact with air/moisture, so what is the procedure for this?

23 January 2021 428 2 View

Is it possible to mix TCEP-HCl and NaI chaotropic reagent?

I have a lack of knowledge on sample preparation for RNA extraction to test molecular diagnosis. I just want to know whether it is plausible to mix TCEP-HCl lysis/reducing buffer and NaI...

21 January 2021 9,309 1 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How Can we Avoid Overfitting easily in machine learning?

29 January 2021 9,307 6 View

How to control the voltage fluctuation? PV panel voltage fluctuation controlling method ?

As we know due to the fluctuation of solar irradiance the terminal voltage is also fluctuated which is not acceptable and we may control this fluctuation by controlling the grid side inverter.

19 October 2020 4,459 3 View

The fluctuation in the teaching process from traditional to e-learning and versa. Is it a feasible process for teaching?

The fluctuation in the teaching process from traditional to e-learning and vice versa. Is it a feasible process for teaching?

23 September 2020 8,522 97 View

How to analyze unbalanced longitudinal data?

Most of recent books in longitudinal data analysis I have come through have mentioned the issue of unbalanced data but actually did not present a solution for it. Take for example: Hoffman, L....

25 May 2020 3,041 9 View

Different types of clouds have different impacts on the Earth's climate?While some types of clouds help to warm the Earth, others help to cool it,How?

Currently, the combined effect of all clouds is one of net cooling, meaning that clouds are dampening the rate of climate warming. But scientists are looking into whether clouds will have the same...

09 May 2020 9,367 19 View

Reversed phase C18 coulmn Azobenzene derivative?

Dear all, I need your helpful suggestions to solve one of the issues related to evaluating the purity of an azobenzene type compound using a C18 reversed-phase column. The compound dissolves...

08 May 2020 6,440 3 View

How could I use photodiode to do signal compensation?

Hi all, I am working on building fluorescense flow cytometry now. I've been asking to set a photodiode to monitor the laser status during the system is operating. The reason is the laser might...

31 March 2020 5,792 2 View

What would be the X-ray diffraction intensity of a ternary compound with one atom spatially fluctuating inside the octahedra?

If a ternary ABC2 compound with two sublattices, say BC4 and AC8, where B and C are at the center of tetrahedra and octahedra respectively, suppose if A atom is randomly fluctuating inside the AC8...

23 January 2020 4,770 4 View

What would be the X-ray diffraction intensity of a ternary compound with one atom spatially fluctuating inside the octahedra?

If a ternary ABC2 compound with two sublattices, say BC4 and AC8, where B and C are at the center of tetrahedra and octahedra respectively, suppose if A atom is randomly fluctuating inside the AC8...

31 December 2019 2,130 4 View