Cleaning up single cell RNA from 10x dataset?

Hello, I am new to single cell RNA data processing and am currently running my data through the initial cleanup steps via Seurat on R. I have previously done this on epithelial organoid data and...

23 December 2020 4,709 1 View

Fluorimeter giving odd results for carbon quantum dots?

I made a Pb sensor using carbon quantum dots (CQDs). I add buffer, CQDs, and a given metal to a quartz cuvette of the recommended type. Before adding a metal, the cuvette will emit a yellow-green...

16 December 2020 9,675 3 View

Is the fluorescent protein must be totally photobleached in FRAP assay?

Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...

14 December 2020 6,567 1 View

Recommend resources for developing surveys of technology-users for "needs-analysis" or design criteria?

I am looking for recommendations for good resources or guidelines for developing surveys specifically focused on targeting user's opinions or a needs-analysis in the context of engineering design...

12 December 2020 6,698 6 View

Can an algorithm come into control and possession of data without the knowledge of or input from a human programmer?

Keeping in mind the adage on machine learning: data in, data out. I am curious whether there are neural network algorithms that could allow an algorithm to control and possess data on its own...

30 November 2020 860 11 View

Which is the best Instrument for obtaining the geophysical properties of sub-soil?

For performing the real scenario ground seismic simulation, we require certain input parameters like Shear wave variation with depth or Vs (30), density of the soil layers etc. Following...

29 November 2020 9,714 4 View

How much salt in a m^3 of water would be needed to prevent evaporation ?

Assume you have 1 cubic meter of water with 1 square meter of surface exposed to the sun. If you kept adding salt to the solution, is there a point at which evaporation would cease? Assuming the...

22 November 2020 5,444 1 View

Protein concentration decrease after dialysis?

Recently I have been involved in this problem: After expressed by adding IPTG, lysed with B-PER, and resolved with Buffer B (8M urea), incubate with Ni-NTA beads, everything looks fine. When I...

21 November 2020 7,416 3 View

What is the difference between S8R and S8R5 element in Abaqus for in-plane loading?

I understand that S8R is for thick shells, and S8R5 is for thin as well as their inherent plate theories. However, in the case of an in-plane loading such as a bias-extension of a thin composite...

02 November 2020 5,105 1 View

Removing of sticky phases of cement from alumina crucible ?

How to remove the sintered material which has been fully stuck at the base of alumina crucible? It is synthesized as stone like material after sintering at 1400 deg C. Should any other kind of non...

28 October 2020 6,380 2 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

What is the best solvent to remove PDMS from nano particles?

I want to remove PDMS oligomers from the surface of nanolayers. I have just read a very helpful paper about the compatibility of PDMS with different solvents( Solvent Compatibility of...

24 February 2021 7,845 5 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How to interpret this FTIR spectrum of pyrolyzed hydrochar?

20 February 2021 2,840 3 View

How to determine number concentration of polymer solutions using viscosity experiment?

I have synthesized PNIPAM (Poly (N-isopropylacrylamide)) microgels, which are copolymerized with oligo-ethylene glycol based comonomers. I have to determine the exact number concentration for each...

19 February 2021 5,205 5 View

What is error #2070 in gaussian.how we do resolve it?

We want to ONIOM calculation in gaussian16. However, gaussian doesn't work. What should we do? Atom 15 layer R linked to atom 85 is also bonded to atom 161 in layer M bad...

15 February 2021 7,766 3 View

How can I convert permeability unit from barrer to m^2 for using COMSOL MultiPhysics?

I am looking for gas permeability of PDMS for simulation in COMSOL MultiPhysics and using creeping flow , fluid and matrix properties. The permeability unit in COMSOL is m^2 whereas I have the...

08 February 2021 4,305 2 View