Can you suggest any recent papers explaining the causes of hyperhydricity in tissue cultured explants?

What exactly IS hyperhydricity? What are the micro/macroscopic characteristics of hyperhydric explants? How does hyperhydricity spread through an explant? Can hyperhydricity be caused through...

16 December 2018 5,790 2 View

How do you decide what species to include in phylogenetic tree for paml positive selection analysis?

Hi, I am trying to test if one copy of a duplicated gene is under positive selection in 1 particular fish species. The other copy of the duplicated gene has been observed to be under positive...

04 September 2018 1,489 1 View

I have been performing simultaneous TGA/DSC runs on a polymer. I am getting a 60% mass loss but no exo/endothermic reaction to accompany. Any ideas?

The reaction takes place routinely at 550C with no change in energy. Has anyone experienced this before?

18 December 2017 4,207 13 View

How do you measure the imaginability of writing?

20 October 2017 5,928 4 View

How do you measure the imaginability of writing?

20 October 2017 2,616 5 View

Can I reuse primary and secondary antibodies for immunohistochemistry?

In a lab I rotated in, we always reused primary antibody but never reused secondary. In the lab I've chosen to remain in, it is considered okay to reuse secondary antibodies. I am a bit hesitant...

27 September 2017 3,822 7 View

What quantitative instruments exist for analysing children's writing?

15 February 2017 9,692 5 View

What fluorophore should I use in my FRET analysis of liposomes containing the carotenoid pigment zeaxanthin (or lutein)?

I am creating nanoliposomes to use as a delivery method to mix hydrophobic pigment zeaxanthin with hydrophilic protein GSTP1. My faculty adviser wants to use FRET to help measure how well GSTP1...

20 June 2016 3,155 2 View

Literature on Knowledge Management in Educational Institutions

Hi all, I'm currently working on my dissertation and I'm looking for more leads on different journals, books, websites on knowledge management in educational institutions. Your help will be...

01 January 1970 4,599 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View