Giorgia Pertile

4 Questions 193 Answers 0 Followers

Questions related from Giorgia Pertile

Protocol PCR primers narG1950m & narG2050m?

Did someone used these primers in PCR or qPCR? Because I have a problem to perfom the PCR and I obtained the no appropriate band in the samples and NTC. Thanks Giorgia

30 January 2018 1,636 3 View

Which method do I use to find the best gene reference to use un the RT-qPCR?

Hi, I need to find a gene reference to use in the gene expression of cellulolytic genes in Ascomycete. Which gene reference is possible ti use? Or which is the method to find it? Thanks Giorgia

01 June 2017 9,891 1 View

Why did not work the digestion of HaeIII?

Hi, I am working on the T-RFLP and I am trying to optimase this analysis on the ITS regions. For the restriction enzyme, I use the HaeIII enzyme. I tried this reaction in two different way: 1)10...

17 May 2017 1,830 3 View

RT-qPCR protocol one or two steps?

Is best to prepare the RT-qPCR in one-step or two-step?

27 November 2016 9,825 8 View