Hi
I want to label a protein with Rhodamine B Isothiocyanate which failed. I have the protein in PBS pH 7.2 the Rhodamine B Isothiocyanate dissolved in DMSO at 10 mM. added 15 times molar excess of the dye to my protein and incubated it in room temperature for 1h followed by buffer exchange using Zeba spin column. The labeling did not work as determined by SDS page that was analysed in a bio-rad chemi-doc with the rhodamine setting. (also a lot of dye followed through in the spin column but still nothing seems attached). neither my protein not my control BSA was labeled.
Gustav N Sundell
I hope you find good information on the attached article: Stability of bovine serum albumin labelled by rhodamine B isothiocyanate
Ikram Madani Ahmed no that answers no questions actually. The description of how the labeling was done is not at all sufficient to explain anything of what they did. And you know i can also use google i wanted an answer not a google.
Thank you. Your question is still on the gate. I hope you will be provided with a good answers.
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