How to remove unreacted florescent dye from labeled protein?

Hi I have a protein labeled with rhodamine B and after reaction i used Zeba spin column for dye removal. my problem is that lot of unreacted dye remained which interfered with my florescent...

02 February 2021 644 2 View

Protocol for protein labeling using Rhodamine B Isothiocyanate?

Hi I want to label a protein with Rhodamine B Isothiocyanate which failed. I have the protein in PBS pH 7.2 the Rhodamine B Isothiocyanate dissolved in DMSO at 10 mM. added 15 times molar excess...

26 January 2021 7,468 3 View

Best practice for graphing linked data?

In an experiment with cardiomyocytes, each recorded cell was exposed to three different conditions. A) was a basal recording, B) was a low dose of a drug, and C) was a high dose of a drug. Every...

31 August 2020 3,246 1 View

How many nucleotides can you add in a PCR?

Hi so i have before added restriction sites and stop codons by flanking the primer with extra nucleotides. But now the question arises can I add more stuff this way? like a whole His-tag worth of...

24 November 2019 6,230 3 View

How do I bind Graphene to epoxy?

I am looking to use graphene sheets as strain gauges inside of a prepreg composite made of carbon fibers and epoxy resin (or BMI if easier). I haven't decided which kind of...

12 December 2018 6,073 3 View

Can you freeze diluted HRP-conjugated antibody?

Is it possible to reuse HRP conjugated antibody for ELISA after you have frozen(-20) the antibody that is diluted to experimental concentration?

18 May 2016 9,039 7 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View

Are there existing studies in economic sociology that have applied Kalyan Sanyal's theoretical framework as a methodological basis?

To be precise, I am looking for studies on informal labour that have taken on board Sanyal's category of the Need Economy and his 'Politics of Exclusion' in studying the political-economy of...

26 January 2021 4,219 3 View

Membrane protein Expression Purification: Broad Asymmetric peak on size exclusion ?

Hello, I am expressing an integral membrane protein (MW 51kDa) in Hi5 insect cells. I initially purified the protein by Nickel-His, concentrated it and subjected it to Size Exclusion...

10 December 2020 2,781 5 View

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02 December 2020 7,293 2 View

How to prevent protein aggregation in gel filtration (size exclusion) column ?

I am currently trying to run solubilised mitochondria extracts from mammalian cells through a gel filtration column in order to separate proteins by size & visualise protein complexes. My...

29 November 2020 1,483 5 View

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I used triplicate for a sample in qPCR, and the Ct values are 26.0, 26.02 and 31.1 It is obvious that 31 is an outlier which may be resulted by technical error and I want to exclude it in the...

17 November 2020 4,458 3 View

What is the best method to conduct a meta analysis?

Dear Colleagues: For those of you who have conducted a couple of meta analyses, do you agree with these steps? What is the best method of extracting the data from the chosen studies? Formulate...

26 October 2020 1,539 6 View

60KDA contaminant turning protein sample yellow?

I'm working on the purification of a few different his tagged proteins and while some have turned out good, other preps present this strong yellow colour after purification. I've expressed in a...

27 September 2020 4,359 7 View

Excluding responses from a data set based on exclusion criteria?

This question may seem obvious but I am a novice researcher currently completing my MSc dissertation... When analysing responses to my online survey, should I exclude responses which fit my...

15 September 2020 1,096 3 View