if i wanted to collect a clinical sample from healthy individuals of different age groups including older adults, do i need different exclusion criteria for healthy between age groups? What criteria should then be age related?
Hi i have tried through literature search find out how "bendy" m13 phage is. and so fare i have come back with nothing. there are papers describing how much the phage can stretch but nothing...
17 January 2024 2,270 0 View
Most phage display selections are made in 2-5 rounds, meaning that the phages that bind to the target gets amplified through growth in bacteria between each round. But PhIP-seq, which is phage...
19 December 2023 8,532 0 View
Hi i am starting working with insect cell protein expression from previously working with bacterial protein expression. for bacteria you can pellet and then freeze the cells after expression...
10 April 2023 8,295 2 View
hi it seems that my lab has some kind of lytic ohage contamination from some where. does anyone have any good cleaning ideas to get rid of them? we autoclaved the pipetts and cleaned all surfaces...
10 July 2022 6,847 2 View
Is there a way to regenerate streptavidin magnetic beads? I am guessing no because i am guessing when i elute i break the interaction between my biotinylated peptide and the protein not between...
16 November 2021 5,448 3 View
I have neutravidin and want to immobilize it on 96 well maxisorp plates so i can bind biotinulated peptides to it for elisa. does anyone have a good protocol for this? what buffer, good...
07 October 2021 7,447 1 View
So we have an FPLC system which is the P900 that has been acting wierd for a week or two. Even though no air should be in the system the pump introduces air anyways and makes the clicking noise...
16 March 2021 3,451 2 View
Hi I have a protein labeled with rhodamine B and after reaction i used Zeba spin column for dye removal. my problem is that lot of unreacted dye remained which interfered with my florescent...
02 February 2021 897 2 View
Hi I want to label a protein with Rhodamine B Isothiocyanate which failed. I have the protein in PBS pH 7.2 the Rhodamine B Isothiocyanate dissolved in DMSO at 10 mM. added 15 times molar excess...
26 January 2021 7,752 3 View
In an experiment with cardiomyocytes, each recorded cell was exposed to three different conditions. A) was a basal recording, B) was a low dose of a drug, and C) was a high dose of a drug. Every...
31 August 2020 3,461 1 View
Research being conducted by Stellenbosch University would like your opinion about the effects of social exclusion on emerging urban mobility options and traditional public transport services. By...
21 July 2024 4,032 0 View
I forgot to autozero during the run (Size exclusion chromatography.) and later i realised i forgot to do that and the baseline was not zero but below zero (and in some cases it above zero). I...
15 July 2024 5,551 6 View
I was using Superose6 10/300 Gl column for purification of my target protein. the sample consists of various oligomeric state and i would like to identify the approximate elution volume where my...
15 July 2024 5,157 2 View
I did SDS stainning from P. ostreatus liquid culture. I have tried using Coommassie BR but it doesn't work, then I change to silver stainning. It worked perfectly after vivaspin but when I used...
22 May 2024 3,493 0 View
hi everyone, I have three objectives in the protocol. My first objective is to calculate the incidence of specific illness presentations to the emergency departments. Just to be sure if I have...
06 May 2024 2,208 2 View
I can't find a regeneration protocol for this type of resin specifically, only how to do a cleaning for size exclusion chromatography resins, in a general way.
15 April 2024 8,142 1 View
15 April 2024 8,590 2 View
According to the study of Pan et al. (2023) entitled "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis," what are the...
08 April 2024 3,776 1 View
Current undergraduate here in process of writing a literature review. I have just start the process of developing my inclusion and exclusion criteria for a set of articles that have already...
02 April 2024 7,889 0 View
I am planning to start a study utilizing 810 nm light to stimulate the vagus nerve bilaterally and am looking at exclusion criteria. Thank you.
26 March 2024 9,614 3 View