21 Questions 7 Answers 0 Followers
Questions related from Gustav N Sundell
Hi do anyone know of a standard vector that can express GST without any fusion protein? As I understand it is that for many standard vectors that uses GST as fusion protein doesn't express GST...
2 weeks ago 4,019 1 View
If i want to run an elisa with antigen coated plates and use serum as my analyte and anti-human ab as secondary antibody is it possible to compare the signal to a standard curve of generic/...
2 weeks ago 9,796 3 View
Hi can you purify just the gst from pDest15?
23 June 2025 7,614 1 View
Hi i have tried through literature search find out how "bendy" m13 phage is. and so fare i have come back with nothing. there are papers describing how much the phage can stretch but nothing...
18 January 2024 2,294 0 View
if i wanted to collect a clinical sample from healthy individuals of different age groups including older adults, do i need different exclusion criteria for healthy between age groups? What...
17 January 2024 5,769 0 View
Most phage display selections are made in 2-5 rounds, meaning that the phages that bind to the target gets amplified through growth in bacteria between each round. But PhIP-seq, which is phage...
20 December 2023 8,573 0 View
Hi i am starting working with insect cell protein expression from previously working with bacterial protein expression. for bacteria you can pellet and then freeze the cells after expression...
11 April 2023 8,323 2 View
hi it seems that my lab has some kind of lytic ohage contamination from some where. does anyone have any good cleaning ideas to get rid of them? we autoclaved the pipetts and cleaned all surfaces...
11 July 2022 6,873 2 View
i am very skeptical about the idea that a pipettes life increases if it is put on max after use. is here any actual data on this?
01 December 2021 1,905 0 View
Is there a way to regenerate streptavidin magnetic beads? I am guessing no because i am guessing when i elute i break the interaction between my biotinylated peptide and the protein not between...
17 November 2021 5,475 3 View
I have neutravidin and want to immobilize it on 96 well maxisorp plates so i can bind biotinulated peptides to it for elisa. does anyone have a good protocol for this? what buffer, good...
08 October 2021 7,476 1 View
So we have an FPLC system which is the P900 that has been acting wierd for a week or two. Even though no air should be in the system the pump introduces air anyways and makes the clicking noise...
17 March 2021 3,478 2 View
Hi I have a protein labeled with rhodamine B and after reaction i used Zeba spin column for dye removal. my problem is that lot of unreacted dye remained which interfered with my florescent...
03 February 2021 934 2 View
Hi I want to label a protein with Rhodamine B Isothiocyanate which failed. I have the protein in PBS pH 7.2 the Rhodamine B Isothiocyanate dissolved in DMSO at 10 mM. added 15 times molar excess...
27 January 2021 7,806 3 View
Hi so i have before added restriction sites and stop codons by flanking the primer with extra nucleotides. But now the question arises can I add more stuff this way? like a whole His-tag worth of...
25 November 2019 6,526 3 View
Is it possible to reuse HRP conjugated antibody for ELISA after you have frozen(-20) the antibody that is diluted to experimental concentration?
19 May 2016 9,196 7 View
Is there a way to dissolve anhydrous ampicillin in water to normal stock concentration? I want 100 mg/ml stock and it did not dissolve. This is the ampicillin i have so don't give me the...
01 January 1970 8,059 3 View
hi Im gonna do a phage display selection with a membrane protein that is detergent stabilized. does anyone have a protocol for this or any experience in doing this? are the phages effected? how...
01 January 1970 1,776 2 View
Hi i am doing phage display and in the amplification stepr growing bacteria dubbelinfected with M13 mibrary and VCSM13 helper phage in 200 ml SB media (10g mops 20g yeast extract 30g tryptone /...
01 January 1970 2,350 0 View
Hi we performed a dilution series 1:10 of different amplifications of the same phage library in Xl-blue bacteria, and we see a strange pattern. In the first dilutions of the reamplified library...
01 January 1970 988 1 View
i wonder if there is anyone that has analyzed the primary sequence distances involved in discontinuous/structural/conformational antibody epitopes? like how many conformationally based...
01 January 1970 5,538 0 View
