Gustav N Sundell

11 Questions 4 Answers 0 Followers

Questions related from Gustav N Sundell

Lytic phage contamination?

hi it seems that my lab has some kind of lytic ohage contamination from some where. does anyone have any good cleaning ideas to get rid of them? we autoclaved the pipetts and cleaned all surfaces...

11 July 2022 6,803 2 View

Does anyone have a protocol for imobilization of neutravidin in maxisorp 96 well plates?

I have neutravidin and want to immobilize it on 96 well maxisorp plates so i can bind biotinulated peptides to it for elisa. does anyone have a good protocol for this? what buffer, good...

08 October 2021 7,409 1 View

Äkta FPLC p900 pump introduces air and clicks, how can I get rid of this?

So we have an FPLC system which is the P900 that has been acting wierd for a week or two. Even though no air should be in the system the pump introduces air anyways and makes the clicking noise...

17 March 2021 3,314 2 View

How to remove unreacted florescent dye from labeled protein?

Hi I have a protein labeled with rhodamine B and after reaction i used Zeba spin column for dye removal. my problem is that lot of unreacted dye remained which interfered with my florescent...

03 February 2021 813 2 View

Protocol for protein labeling using Rhodamine B Isothiocyanate?

Hi I want to label a protein with Rhodamine B Isothiocyanate which failed. I have the protein in PBS pH 7.2 the Rhodamine B Isothiocyanate dissolved in DMSO at 10 mM. added 15 times molar excess...

27 January 2021 7,661 3 View

How many nucleotides can you add in a PCR?

Hi so i have before added restriction sites and stop codons by flanking the primer with extra nucleotides. But now the question arises can I add more stuff this way? like a whole His-tag worth of...

25 November 2019 6,343 3 View

Can you freeze diluted HRP-conjugated antibody?

Is it possible to reuse HRP conjugated antibody for ELISA after you have frozen(-20) the antibody that is diluted to experimental concentration?

19 May 2016 9,121 7 View

How to dissolve anhydrous ampicillin in WATER?

Is there a way to dissolve anhydrous ampicillin in water to normal stock concentration? I want 100 mg/ml stock and it did not dissolve. This is the ampicillin i have so don't give me the...

01 January 1970 7,998 3 View

Does anyone have experience with proteins in detergent in Phage display?

hi Im gonna do a phage display selection with a membrane protein that is detergent stabilized. does anyone have a protocol for this or any experience in doing this? are the phages effected? how...

01 January 1970 1,690 2 View

Bacterial pellet is a bit orange /pink and little slimy after phage amplification, why?

Hi i am doing phage display and in the amplification stepr growing bacteria dubbelinfected with M13 mibrary and VCSM13 helper phage in 200 ml SB media (10g mops 20g yeast extract 30g tryptone /...

01 January 1970 2,292 0 View

Titration of Phage library colony number increase with further dilution?

Hi we performed a dilution series 1:10 of different amplifications of the same phage library in Xl-blue bacteria, and we see a strange pattern. In the first dilutions of the reamplified library...

01 January 1970 932 1 View