Most phage display selections are made in 2-5 rounds, meaning that the phages that bind to the target gets amplified through growth in bacteria between each round. But PhIP-seq, which is phage immunopreciptitstion and sequencing, the phages that binds to the serum antibodies and gets precipitated using usually proteinA/g magnetic beads and then immediately sequenced through NGS. How come there are no sequencial rounds in PhIP-seq and what would happen if you amplify the phage and did a second round of precipitation?