Blood sample was used to isolate cancer cells by AC electric field. Isolated cell suspension was then cultured in RPMI 1640. After few days moving round objects were found and keep increasing in their numbers. It is possible that during isolation process there might be contamination from surrounding workplace. I had sterilised the equipment with 0.5% Virkon for 5 min before isolation. Is it possible that there will be contamination during blood collection or sample transfer? Just want to have an idea which moving objects they could be?

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