In our lab. we have 2 versions of cell culture incubator, one is an old fashion without oxygen controlled, the another is new designed with tri gases; nitrogen, oxygen and CO2.
Will the new version with oxygen monitoring have a better condition for cell growth?
The only benefit which I know is tri gases incubator can do hypoxic condition for cancer cell study.
Any comments of this tri gases incubator?
It may come in handy if your lab or a collaborating lab is looking into respiratory research, oxidative stress/aging (exposure to hyperoxia), research involving stem cells that prefer a hypoxic environment...
Hi Sittisak,
Both the incubator will have nitrogen (most abundant in your lab) CO2 (from growing cells and for maintaining pH of media and O2 for growth of cells. Most cell cultures can be done in your old incubator and you can also use the triple gas incubator for routine work. Your old incubator will have just a CO2 cylinder has only CO2 sensor and relies on N2 and O2 present in your culture room, whereas the triple gas cylinder will have fixed ratio of the three gases and also the incubator has sensors for O2 and Co2.
The most important use of Triple gas incubators is for embryo culture in IVF, as embryos are very sensitive to levels of CO2 and O2, triple gas incubators monitors and maintains the set percentage of each gas. Since any change will result in abnormalities in embryo.
Hope this helps.
Regards,
Prasad
Thank you for all comments.
For me likely that both old and new fashion incubator have the same condition for normal cell culture except we need to have special condition for specific purpose.
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