Ru-Jeng Teng

3 Questions 442 Answers 0 Followers

Questions related from Ru-Jeng Teng

A question about western blot?

I have run western blots for 25 years. Today I got this strange blot using Aviva OAPB01225 klotho antibody and 1% BSA in TBST to block for 1 hour. It doesn't matter whether I load 10 ug or 50 ug...

01 January 2020 9,732 3 View

Loading control for membranbe proteins?

I need to study cell membrane protein by western blots but encounter a problem with adequate loading control. Any suggestions?

08 August 2019 3,220 5 View

What is the effect of N-glycosylation on protein mobility in western blot?

I treated cells with tunicamycin to de-N-glycosylate and found most proteins moved faster in western blot except one protein which moved slower. Is it possible?

11 November 2016 7,579 3 View