Stuart Iain Jenkins

9 Questions 47 Answers 0 Followers

Questions related from Stuart Iain Jenkins

Is immunostaining for macroH2A.1 a good way to differentiate male and female (sex, gender) rat cells in co-culture? Stains only female cells?

I want to identify individual male and female rat cells in co-culture (microglia, astrocytes). Ideally, want technique compatible with further immunostaining. In situ hybridisation seems overly...

09 September 2015 8,702 1 View

Can antibodies / immunoglobulins cross the intact blood-brain barrier (BBB) in 'healthy' humans / rodents?

Antibodies are found in the central nervous system in disease/injury states (e.g. multiple sclerosis). But, do immunoglobulins cross the blood-brain barrier independently, or do invading plasma...

03 March 2015 1,009 5 View

Which microglia genes are appropriate for qPCR or RT-PCR normalisation in cells challenged with LPS (lipopolysaccharide/endotoxin)?

I am investigating changes in microglial gene expression, using qPCR (real time, reverse transcription). Microglia will be challenged with LPS while I establish the protocol, then various...

02 February 2015 9,017 10 View

Are there any standardised methods or general principles for validating in vitro assays as being representative models of in vivo?

I'm trying to assess the utility of various in vitro assays/models, and I'm looking for *general thoughts/opinions* here. Elsewhere, I'm looking at the specifics of modelling the brain, modelling...

08 August 2013 943 1 View

Is anybody using Alvetex 3D cell culture scaffolds? What are your experiences?

Alvetex is a porous polystyrene block in which cells can be cultured. Cells are purported to adopt more biologically relevant morphologies and behaviours. I like the theory, but has anybody tried...

03 March 2013 3,559 1 View

Are there petri dishes which are less than 10 mm in height available?

I need a petri dish, 60 mm diameter or less, with height less than 10 mm. I need to use an upright microscope to image the samples in a petri dish (ideally 60 or 35 mm diameter), but I have an...

03 March 2013 8,535 8 View

Is Olig1 expressed in any non-oligodendroglial lineage cells (NSC, OPC, oligodendrocyte)?

Furthermore, could such expression in other cell types affect oligodendrocyte precursors (OPCs) through some downstream pathway?

01 January 2013 8,975 3 View

Does anyone have any experience of PEG-coated fluorescent magnetic particles or nanoparticles? Specifically, cellular uptake of such particles?

I want to label cells with fluorescent particles, and study the influence of various coatings/surface properties on extent of uptake. I need a commercially available polyethylene glycol...

12 December 2012 3,585 6 View

iZON qNano particle analyser - is anyone using this device?

Does anyone have any experience of using this device (qNano) to characterise nanoparticles? In particular, I'm interested in characterising iron oxide-based particles, with surfaces including...

10 October 2012 3,384 8 View