Sittisak Pui-Ock

10 Questions 29 Answers 0 Followers

Questions related from Sittisak Pui-Ock

Is the oxygen level monitored in the incubator important?

In our lab. we have 2 versions of cell culture incubator, one is an old fashion without oxygen controlled, the another is new designed with tri gases; nitrogen, oxygen and CO2. Will the new...

05 May 2015 4,111 4 View

Where might one buy particulate matter (PM) 2.5 um?

We are looking for PM 2.5 for our animal model study. So far, I can not find commercial particulate matter 2.5, most available in the market are DEP or urban particulate matter which is a variety...

11 November 2014 2,215 10 View

How much and what kind of Matrigel is needed to coat a plate?

I plan to grow isolated circulating tumor cells (CTCs) on a Matrigel-coated well plate. I have learned that there are 2 types of Matrigel-coated well plates and that one is thick and the other...

04 April 2014 7,681 6 View

Health impact of autogas, LPG fueled car?

Currently, here in Thailand we are using a lot of cars fueled with LPG as an alternative source of energy. Since its price is cheaper than gasohol and diesel, increasingly, people turn their cars...

11 November 2013 650 2 View

Does anyone have experience using a 3D cell culture novel screening method for anticancer agents?

3D cell culture in liquid form. This is quite new to me. Usually, I always heard about 3D collagen gel model for cell profiration or migration. In this new case they (Nissan Chemical Industries)...

07 July 2013 5,963 3 View

What is a good performance for Cytocentrifuge?

We are looking for a brand new cytocentrifuge which will be used for cell sedimentation and fixation. Since this is very new to me, are there any comments or criteria should I have to...

04 April 2013 7,048 4 View

Any comments on circulating tumor cell isolation by size, a commercial product.

I have learnt from a website who make a cell isolation product named ScreenCell that it can be used to isolate tumor cells circulating in blood current by filtation. It seems to me that this...

02 February 2013 5,970 28 View

What type of cell is it? (image attached)

I have a question on cultured cell staying in RPMI medium for a month without an idea what is it. Some informations are; Source of sample: blood sample from cancer patient. Separation method:...

12 December 2012 3,926 20 View

Is it possible to culture circulating tumor cells?

We are developing a technique that can isolate circulating tumor cells (CTCs) from patient blood. I wonder if we can culture isolated CTC cells starting from very few cells since normally there...

11 November 2012 6,215 7 View

Moving objects found in cell culture of isolated cells. What could they be?

Blood sample was used to isolate cancer cells by AC electric field. Isolated cell suspension was then cultured in RPMI 1640. After few days moving round objects were found and keep increasing in...

10 October 2012 248 12 View