Do you have any suggestions for me to modify my LS-DYNA model?

Hi, I use LS-DYNA to simulate the steel composite shear wall now. I use MAT_084/085 to simulate concrete, MAT_159 to simulate steel plates, and MAT_024 to simulate studs. The concrete section...

24 January 2021 3,863 1 View

Why there is a band around 25Kd of caspase3 in both undifferentiated and differentiated PC12 cells?

Dear all, When I detect the protein expression of caspase3 in PC12 cells, there is always a band around 25Kd. According to literature, the pro-caspase3 should be 35Kd and the cleaved caspase 3...

21 September 2020 8,577 2 View

How to claim back your publication?

My publications have been wrongly claimed by other ppl and I could not claim it back. Browsed through all the pages/tabs, but still could not find where to make the complain. Anyone knows how to...

19 August 2018 7,538 13 View

Does anyone have information about plane failure on rock slope?

07 April 2016 8,054 3 View

What is the NRTL model in chemical engineering?

I'm wondering what is the NRTL model and its usages. I wanna know about NRTL HOC, as well. Please let me know. Thanks.

27 June 2015 5,427 3 View

How to get the standard redox potential of CO2 + 6H+ + 6e- -CH3OH + H2O -0.38 V vs NHE at pH7?

08 February 2015 2,269 3 View

Is it possible to measurement Ca2+ concentration in neurons using a microplate reader?

I want to use fluo-3 to detect the intracellular Ca2+ and I used spectramax M5. Can I use serum-free medium as the buffer?

18 December 2014 4,361 3 View

How to set the parameters for valence band XPS checking?

Normally, we only check the survey XPS and high resoulution XPS of different elements. How to check the valence band XPS? can we check the XPS VB by seting the survey scan range from 20 eV to -5...

01 January 1970 9,086 7 View

How to respond to biased reviews

It has been almost 15 years since my first submission of a manuscript for journal publication. Some of my recent experience, grateful and furious, makes me decide to write such a post. After...

01 January 1970 2,213 6 View

How can Virtual Reality be applied for pediatric imaging and radiology?

Virtual reality is an emerging and essential technology in computer science. It is also useful for medical cares such as surgical simulation and understanding viruses. But how could the virtual...

01 January 1970 6,274 3 View

Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,090 3 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

What questions should i include in my questionnaire?

I am writing my dissertation, about adults who trained at elite status in their youth, i am constructing a questionnaire with the goal of seeing, how many sustained injury, how many sustained...

28 January 2021 4,787 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View

Where did Mitoprot software go (download version)?

Hi all! I've been using local copy of Mitoprot software for prediction of gene localization. https://ihg.gsf.de/ihg/mitoprot.html I've updated my laptop and now need to download Mitoprot again,...

18 January 2021 4,368 3 View

How does a cell repair itself? Can you relate this to mathematical genetic algorithm?

I am on a quest to solve how a cell repairs itself through encoding-decoding of proteins. Is there any link to genetic algorithms to solve age old questions such as aging and how we heal?

14 January 2021 9,958 1 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling?

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling? Any thoughts on applicable small molecules to...

06 January 2021 4,324 2 View

What's the reasonable loading control of nuclear protein in acute injury?

What's the reasonable loading control of nuclear protein in acute injury? Is HDAC1 or Histone H3 a resonable loading control of nuclear protein in acute injury model? How to explain total my total...

06 January 2021 1,607 2 View

Does anyone have any unreported cases of the Barakat (HDR) syndrome?

Barakat (HDR) syndrome is characterized by the triad of Hypoparathyroidism, sensorineural Deafness and Renal disease. In 2018 I published a paper entitled "Barakat AJ, Raygada M, Rennert OM....

29 December 2020 8,647 2 View