Dear colleagues and scientists:
I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of acute kidney injury. Is the use of drugs known to induce kidney injury remains the best option, or should we use other inducers? And does HEK293T expresses the needed biomarkers of injury such as TNF-alpha, interleukins, KIM-1, and others? Thank you for your help.
Hello Alexis Labrador
The below attached article will be helpful!
Article Tissue Culture Models of Acute Kidney Injury: From Tubule Ce...
Best.
A major discussion in the field is the use of appropriate models of tubular cells. Tubular segments are different along its axial distribution, different in energy production, metabolism, cell biology and susceptibility to injury insults. First, check the objective, if it is to study i.e. proximal tubules, you should go for accepted cellular models, such as immortalized cell lines (LLC-PK1 cells, OK cells, HK-2 cells) or primary. the method to mimick AKI should be check as well. Hypoxic treatments as well as drugs that disrupt the mitochondrial energy production complex are well accepted for this matter, along with drugs that induce nephrotoxicity in vivo, such as Doxorubicin, Aminoglycosides (Gentamycin), among others.
Thank you, Malcolm Nobre, for the recommendation; I am reading this paper now. Thank you, Rodrigo Pacheco Silva-Aguiar, for elaborating on which cellular model to use, which depends on the study's objective.
All these help improve our study.
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