Species identification.Who can tell me the scientific names of these Gastropods?

Species identification. I've been doing research on communities of marine macrobenthos recently, but some of them can't be identified, which hinders my research. Who can help me identify these...

14 December 2020 8,357 3 View

I alway get serval big plaques even with very high concentration of bacteriophages. how to get well-distributed plaques with double layer agar method?

I used MS2 (ATCC 15597-B1) strain to do my experiment. I always get serval big plaques (like the picture I attached), but not a plate which has a lot of small plaques I saw from other research....

15 November 2020 7,488 4 View

How can I make the Gold-Antibody conjugate stable ?

I did competitive lateral flow immunoassay using gold bridge method. Antigen-fluorescence bead and immobilized antigen on the PMMA membrane is no problem to measure the optical absorption at 37...

24 August 2020 2,684 4 View

How to represent damping in a system with nonlinear stiffness?

We all know that damping can represent by Damping ratio and Natural frequency . We can represent the damping in terms of ( ''Linear stiffness - The first term in the nonlinear stiffness )...

25 May 2020 6,323 7 View

RNA extraction methods for virus w/o centrifuge?

20 April 2020 4,990 3 View

Anyone has experience in "ligation-free in vivo DNA assembly in E. coli"?

There is a recent publication titled "In vivo DNA assembly using common laboratory bacteria: A re-emerging tool to simplify molecular cloning" (J. Biol. Chem, 2019; doi:...

05 March 2020 153 3 View

How to express and purify Zinc-binding proteins?

We are working on a protein that is predicted to contain a zinc finger (ZnF). Could someone share your experience working with ZnF proteins? Should we add Zn during expression in E. coli? If so,...

23 February 2020 2,125 1 View

A few questions about neoantigens for cancer therapy?

In trying to getting more effective neoantigens for cancer therapy, I have some questions as followings. 1. T-cell activation is considered to be more important than B-cell activation. Which...

01 February 2020 5,667 1 View

How to characterize formation of semi-interpenetrating polymer network (IPN) structure?

Now I am working on synthesize semi-IPN hydrogel based on polysaccharide and vinyl based monomers. Is there any direct characterization method to confirm formation of the semi-IPN structure?...

15 January 2020 2,269 1 View

Why do the recrystallized grains produced by annealing after cold working have different orientations?

09 May 2019 9,420 2 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

How long does it take to get a modeller(for homology modelling) license?

I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...

25 February 2021 9,054 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

What Model for Vaporization and Condensation in Fluent will be best?

Hello, I am running a simulation that includes water phase changes. These phase changes will take place below the boiling point of water so I need a method that can accurately model this. From my...

15 February 2021 4,973 3 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

How can I find the thermophysical properties of high temperature microencapsulated phase change materials (MPCM)?

Hi everyone. I am looking for a high temperature MPCM. More specifically, I need the thermo physical properties of a microencapsulated phase change material with the melting temperature between...

14 February 2021 3,606 2 View

Post evaluation of close range photogrammetry experiments ?

I am performing a simple close range photogrammetry test and comparing outcomes of photogrammetry tools such as VisualSFM, Meshroom, Colmap etc. Kindly advice, what post evaluation tests/ criteria...

13 February 2021 6,410 3 View