Greetings, dear colleagues! Do you know any technique utilizing some ~ordinary molbiol lab reagents (not the commertial kits for isolated mitochondria staining) that will allow isolated mitochondria staining? Maybe SYPRO Ruby will work, since mitochondria do possess a high protein amount? DAPI to stain mitochondrial DNA (though in Article Copy numbers of mitochondrial genes change during melon leaf...
it didn't work to stain mtDNA, but maybe due to high plastids and nuclear fluorescence?)?Or EtBr (though in Article Hypoxia-induced mitochondrial stress granules
authors emphasize that only stress-induced granules can be stained with EtBr)?Or some dyes that are capable of staining lipids? Rhodamine 6G?
Or maybe the methods of in-gel activity staining would work, e.g. mixing mitochondria with NBT/BCIP substrate and supply with NADH? Will formazan precipitate inside of mitochondria?
Or the TTC test - since mitochondria is enriched with dehydrogenases?
Any ideas?
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