I have used this BIorad Powerpac Basic for 4 years now, before which it was used for another 5-6 years. Generally we addressed the E1 error by compensating with fresh buffer and/or filling the buffer upto the required level, and the powerpac would be back on track. 50V of constant voltage gave me 25-30mA. Only this time, I used fresh buffer and checked the level but the powerpac was running on low current (50V gave me 11mA; constant current of 30mA gave me 200V). Later the powerpac kept showing E1 no matter what voltage I set. Whereas another powerpac with the same gel setup ran with sufficient current.
Please suggest me any means that I can address this issue. Is my powerpac broken or is there an issue with power supply? Can it be repaired?
Hi Srija,
It seems like that the buffer is not the problem as it works fine with another apparatus. And E1 error is basically related to the power supply, so re-checking all the electrical connections, un-plugging and then re-plugging the power cord, tightening the electrodes on the cassette, making sure the cassette (inner buffer chamber) isn't leaking and is filled till the required level might help. I am not sure about any issue about the apparatus' working.
Best,