Do you have any suggestions for me to modify my LS-DYNA model?

Hi, I use LS-DYNA to simulate the steel composite shear wall now. I use MAT_084/085 to simulate concrete, MAT_159 to simulate steel plates, and MAT_024 to simulate studs. The concrete section...

24 January 2021 3,863 1 View

How should I prepare my tissue sample for biodistribution study?

I want to evaluate the biodistribution of an elastin-based nanoparticle drug in tumor, heart, liver, and kidney for the first time. Can anyone give me any idea about how to prepare my tissue...

04 January 2021 3,661 3 View

How to calculate the normalized frequency V of photonic crystal fiber using COMSOL?In particular, how to calculate NEFF and nFSM using COMSOL?

24 November 2020 967 2 View

Hi every one. Can any body help me in finding PCR based markers for rice fertility restorer genes in R-line?

I need PCR based markers related to fertility restorer genes in R-line of rice hybrid, fertility maintainer genes in B-line of rice and cytoplasmic male sterility genes related markers in -line.

17 November 2020 4,193 1 View

What type of model for Interclass Correlation Coefficient (ICC) analysis should I use to check reliability of test-retest data?

I measured a Psychometric parameter for 60 participants. I collected the data thrice, so I acquired 3 values for that parameter, of which I calculated the mean value. Since the data was relative...

25 October 2020 5,075 3 View

Should you report quality of individual studies in rapid or systematic reviews?

I am sure that I have read guidance that reporting individual quality of studies in reviews is not good practice, but that it is better to report quality of evidence statements (which are based on...

12 October 2020 7,075 6 View

How do I change to make an insoluble recombinant protein to a soluble protein?

How do I change insoluble recombinant proteins to soluble proteins? My recombinant protein is Aldose dehydrogenase from bacterial with around 35.0 kDa size. Now, My expression vector and...

05 October 2020 1,840 2 View

Why there is a band around 25Kd of caspase3 in both undifferentiated and differentiated PC12 cells?

Dear all, When I detect the protein expression of caspase3 in PC12 cells, there is always a band around 25Kd. According to literature, the pro-caspase3 should be 35Kd and the cleaved caspase 3...

21 September 2020 8,577 2 View

mScarlet Elisa Detection?

I've been trying to detect mScarlet protein purified from E. coli via an ELISA assay. My protein has two tags: an N-terminal Strep-tag and a C-terminal His-tag. To do the ELISA I've used...

20 September 2020 730 2 View

Phd Research Suggestion on Geo technical Engineering?

Phd Research Suggestion on Geo technical Engineering?

05 July 2020 202 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View