Pratibha Srivastava

5 Questions 51 Answers 0 Followers

Questions related from Pratibha Srivastava

JMP Pro 14 and one way ANOVA?

I have to analyse the data for one way ANOVA using JMP pro 14 software. Anyone dealt with this software or has any kind of experience. Kindly share it. Thanking you in advance.

24 September 2020 685 6 View

Can anyone explain me how to design tet-on and tet-off system in mouse and human cell line?

Is there a different methodology in mouse and human expression. I need to check the expression of a gene under the influence of two proteins. Thanking you.

25 January 2019 8,260 1 View

Why do DH5 alpha express different length of transformants from a same ligated product?

I experienced a slightly different transformants length of my ligated product. They maybe varied by less than 100bp in length!

13 December 2018 5,932 2 View

Why do the mutants aren't expressed in the 293 cells whereas Full-length WT construct is expressed ?

I prepared the recombinant protein X-FLAG tagged and two of its mutant. I have checked the mutant was correctly assembled in the pShuttle vector but cannot discover its expression even after 1...

11 December 2018 406 16 View

I want to prepare an overexpression module for my protein with a N-ter HA tag. Can you suggest me the possible strategies?

I have tried by breaking the protein into multiple fragment and using the PCR products to clone one by one. I successfully cloned into the pShuttle vector but I couldnt get the expressed protein,...

27 February 2018 9,967 2 View