Do you have any suggestions for me to modify my LS-DYNA model?

Hi, I use LS-DYNA to simulate the steel composite shear wall now. I use MAT_084/085 to simulate concrete, MAT_159 to simulate steel plates, and MAT_024 to simulate studs. The concrete section...

24 January 2021 3,863 1 View

Why there is a band around 25Kd of caspase3 in both undifferentiated and differentiated PC12 cells?

Dear all, When I detect the protein expression of caspase3 in PC12 cells, there is always a band around 25Kd. According to literature, the pro-caspase3 should be 35Kd and the cleaved caspase 3...

21 September 2020 8,577 2 View

Is there any open source code for learning in the field of data-driven robust optimization?

I can get a lot of theoretical knowledge in related fields, but I'm still confused about the implementation behind it. Unfortunately, I didn't find any examples on the Internet that I could...

29 March 2020 6,438 3 View

How to calculate intersystem crossing rate from Dalton?

Dear all, I'd like to calculate the intersystem crossing rate using Dalton package, for example, from S1 to T1. Now I can only get the spin orbit coupling matrix element (SOCME), but don't know...

21 August 2019 6,595 4 View

Does anyone have information about plane failure on rock slope?

07 April 2016 8,054 3 View

What is the NRTL model in chemical engineering?

I'm wondering what is the NRTL model and its usages. I wanna know about NRTL HOC, as well. Please let me know. Thanks.

27 June 2015 5,427 3 View

How to get the standard redox potential of CO2 + 6H+ + 6e- -CH3OH + H2O -0.38 V vs NHE at pH7?

08 February 2015 2,269 3 View

Is it possible to measurement Ca2+ concentration in neurons using a microplate reader?

I want to use fluo-3 to detect the intracellular Ca2+ and I used spectramax M5. Can I use serum-free medium as the buffer?

18 December 2014 4,361 3 View

How to improve the concentration of PCR product?

my PCR product only 3ng/ul

01 January 1970 7,191 3 View

How to set the parameters for valence band XPS checking?

Normally, we only check the survey XPS and high resoulution XPS of different elements. How to check the valence band XPS? can we check the XPS VB by seting the survey scan range from 20 eV to -5...

01 January 1970 9,086 7 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

What software is capable of opening a file with the extension ".cad" ?

It seems like quick cad was the software but had been discontinued. I tried with other Autodesk software such as fusion 360, AutoCAD. I also tried with QCAD. Please let me know if you know of any...

22 February 2021 9,634 3 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View