When you fuse tdTomato with scFv to make a fluorescent fusion protein, is it better to use a flexible linker (like GGGGS) or a strict linker?

10 January 2023 0 6K Report

When you fuse tdTomato with scFv to make a fluorescent fusion protein, is it better to use a flexible linker (like GGGGS) or a strict linker? Does the large size of tdTomato affect the binding of scFv?

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