9 Questions 73 Answers 0 Followers
Questions related from Muhammad Imran
Can anyone please share the method for total isothiocyanate determination in food and urine samples? Thanks.
05 May 2015 4,980 2 View
I want to activate Akt in human myeloid leukemia cells and insulin treatment is one of the best choices for its activation (Akt phosphorylation). Has anyone ever tried insulin treatment in human...
10 October 2013 8,061 8 View
Suspension cells are hard to transfect, thus making it difficult to overexpress or knockdown a gene. The alternative strategies are either to do electroporation, nucleofection or lentiviral...
09 September 2013 5,245 42 View
While detecting the phospho-protein people block their NC or PVDF membranes with BSA (3-5%) rather than NFM. Can any one please explain why BSA blocking is preferred for detection of...
07 July 2013 1,492 15 View
List of N-Ras mutant cells....
07 July 2013 6,470 7 View
Cancer cells have different abnormalities, like mutation or depletion of tumor suppressor proteins, as well as oncogene activation. In many cases cancer cells have amplified genes which make them...
07 July 2013 4,296 2 View
Does anyone know the best method for detection of Mycoplasma contamination in the cell culture system? Also what could be the best approach (drug etc.) to avoid mycoplasma contamination?
05 May 2013 714 13 View
I am going to analyze cell surface markers for granulocytic and macrophages differentiation by FACS. what are the specific surface proteins (CDs) that can be asessed?
04 April 2013 1,481 1 View
Can anybody please suggest me the specifc surface proteins (CDs) for asessing the granulocytic and macrophage differentiation of promyleocytic leukemic HL-60 cells?
04 April 2013 8,105 5 View
