Is there anyone who are working on SHSY5Y cell line? I am looking for a donor who can donate the cell line SHSY5Y.

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PVA/ZnO nanocomposite film

03 December 2018 5,459 2 View

Please let me know why my qpcr is not working properly ?...i m very new in qpcr?

I ran qpcr and got strange results like inverted peak in melt curve, improper amplification curve. I repeated the experiment for optimization but everytime i got same error. Then i performed...

10 June 2018 1,443 3 View

Can i use ampicillin for removing Agrobacterium rhizogenes from the transformed plant ?

Can i use ampicillin sodium salt instead of carbenicillin or cefotaxime for selecting transformed plant? i want to use ampicillin as it is cheaper than other antibiotics.... Thank u

09 May 2017 8,875 10 View

How to work with the cancer patients not accepting their condition?

Most of time they will be a state of denial about stages of cancer,chances of severity, even sometime they are reluctant to discontinue their present job. Sometime It perceived like fear,sometime...

04 May 2016 3,048 2 View

What is the maximum non-toxic concentration of DMSO in Cell culture?

19 April 2016 6,934 2 View

Is there any study explain about determination of sample size of RCTs and qualitative designs?

Think you are going to introduce a innovative therapy and you wanted to do a market survey ,what will be your population size and how to determine this.

10 April 2016 8,468 5 View

Calling a variable from main to another function - any thoughts?

I have a main function which outputs image I, J. I need to read these I, J as inputs in another function (comb.m). I have tried declaring I, J as global but when I run comb.m, it's showing...

11 March 2014 3,775 9 View

Image normalization?

I have done a normalization (0 to 1) of an image. I want to display the normalized image but I am unable to. Is this normalized image similar to binary image?

09 February 2014 341 4 View

Can anyone suggest a feasible application of image enhancement?

I did a project on image enhancement using PSO. I have to continue this for my full semester. What improvement is possible?

02 December 2013 3,618 4 View

Why the fluorescence intensity of untreated plus probe control group has high?

Recently, I am performing the intracellular ROS detection by DCF-DA method in SHSY5Y cells through flow cytometer. But, I found a high fluorescence intensity for the untreated control stain with...

01 January 1970 8,661 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View