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PVA/ZnO nanocomposite film

03 December 2018 5,459 2 View

Is there anyone who are working on SHSY5Y cell line? I am looking for a donor who can donate the cell line SHSY5Y.

I will work on Parkinson's disease using SHSY5Y cell line. I have ordered the cell line from NCCS, Pune multiple times. Unfortunately, I have received the cell line contaminated with a slow...

25 July 2017 7,034 2 View

Can i use ampicillin for removing Agrobacterium rhizogenes from the transformed plant ?

Can i use ampicillin sodium salt instead of carbenicillin or cefotaxime for selecting transformed plant? i want to use ampicillin as it is cheaper than other antibiotics.... Thank u

09 May 2017 8,875 10 View

How to work with the cancer patients not accepting their condition?

Most of time they will be a state of denial about stages of cancer,chances of severity, even sometime they are reluctant to discontinue their present job. Sometime It perceived like fear,sometime...

04 May 2016 3,048 2 View

What is the maximum non-toxic concentration of DMSO in Cell culture?

19 April 2016 6,934 2 View

Is there any study explain about determination of sample size of RCTs and qualitative designs?

Think you are going to introduce a innovative therapy and you wanted to do a market survey ,what will be your population size and how to determine this.

10 April 2016 8,468 5 View

Calling a variable from main to another function - any thoughts?

I have a main function which outputs image I, J. I need to read these I, J as inputs in another function (comb.m). I have tried declaring I, J as global but when I run comb.m, it's showing...

11 March 2014 3,775 9 View

Image normalization?

I have done a normalization (0 to 1) of an image. I want to display the normalized image but I am unable to. Is this normalized image similar to binary image?

09 February 2014 341 4 View

Can anyone suggest a feasible application of image enhancement?

I did a project on image enhancement using PSO. I have to continue this for my full semester. What improvement is possible?

02 December 2013 3,618 4 View

Why the fluorescence intensity of untreated plus probe control group has high?

Recently, I am performing the intracellular ROS detection by DCF-DA method in SHSY5Y cells through flow cytometer. But, I found a high fluorescence intensity for the untreated control stain with...

01 January 1970 8,661 2 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

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I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View