Can i use ampicillin sodium salt instead of carbenicillin or cefotaxime for selecting transformed plant? i want to use ampicillin as it is cheaper than other antibiotics....
Thank u
Carbenicillin and cefotaxime are used to kill the Agrobacterium not to select the transgenic plant cells. Ampicillin resistance is common, you should test the Agrobacterium first to see if it is sensitive before you try to kill it with ampicillin
Probably not, in our study >33% isolates of Agrobacterium were resistant to Ampicillin, however as suggested by Susan, you may use after determining sensitivity and MIC of the strains of Agrobacterium present in your area of study.
Timentin (clavulanic acid fortified ticaracillin) is something I've used, and with some strains even vancomycin although not generally as readily available. You could play with supplementing amp with clauvulinic acid as in e.g. Augmentin ... but all of these may well be more expensive than the Carb or Cefo you are trying to avoid. I'd start with the MIC as Bhoj said.
What is the antibiotic your vector confers resistant to (or what is the bacterial selection marker on your vector)? kanamycin, ampicillin or others?
I am interested to know which explant/crop you are transforming and what bacterial OD you maintain in your inoculation experiments. There is possibility of using no or very less amount (less usage if expensive) of car or cefa if explants are properly handled prior tranfering to RSM.
The answer of question asked by Prof. Yuan can lead to few suggestions as well.
Be aware: Different antibiotics (to suppress Agrobacterium) can have different effect on the regeneration abilities of the various plant tissues
- The paper attached demonstrating using different antibiotics to suppress Agrobacterium growth in plant transformation, and the results showed that antibiotics have different effects on the regeneration abilities of the various plant tissues (see yellow highlights)
- The antibiotics they used were: timentin, cefotaxime, carbenicillin and ampicillin
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