Please let me know why my qpcr is not working properly ?...i m very new in qpcr?

I ran qpcr and got strange results like inverted peak in melt curve, improper amplification curve. I repeated the experiment for optimization but everytime i got same error. Then i performed...

10 June 2018 1,443 3 View

Is there anyone who are working on SHSY5Y cell line? I am looking for a donor who can donate the cell line SHSY5Y.

I will work on Parkinson's disease using SHSY5Y cell line. I have ordered the cell line from NCCS, Pune multiple times. Unfortunately, I have received the cell line contaminated with a slow...

25 July 2017 7,034 2 View

Can i use ampicillin for removing Agrobacterium rhizogenes from the transformed plant ?

Can i use ampicillin sodium salt instead of carbenicillin or cefotaxime for selecting transformed plant? i want to use ampicillin as it is cheaper than other antibiotics.... Thank u

09 May 2017 8,875 10 View

How to work with the cancer patients not accepting their condition?

Most of time they will be a state of denial about stages of cancer,chances of severity, even sometime they are reluctant to discontinue their present job. Sometime It perceived like fear,sometime...

04 May 2016 3,048 2 View

What is the maximum non-toxic concentration of DMSO in Cell culture?

19 April 2016 6,934 2 View

Is there any study explain about determination of sample size of RCTs and qualitative designs?

Think you are going to introduce a innovative therapy and you wanted to do a market survey ,what will be your population size and how to determine this.

10 April 2016 8,468 5 View

Calling a variable from main to another function - any thoughts?

I have a main function which outputs image I, J. I need to read these I, J as inputs in another function (comb.m). I have tried declaring I, J as global but when I run comb.m, it's showing...

11 March 2014 3,775 9 View

Image normalization?

I have done a normalization (0 to 1) of an image. I want to display the normalized image but I am unable to. Is this normalized image similar to binary image?

09 February 2014 341 4 View

Can anyone suggest a feasible application of image enhancement?

I did a project on image enhancement using PSO. I have to continue this for my full semester. What improvement is possible?

02 December 2013 3,618 4 View

Why the fluorescence intensity of untreated plus probe control group has high?

Recently, I am performing the intracellular ROS detection by DCF-DA method in SHSY5Y cells through flow cytometer. But, I found a high fluorescence intensity for the untreated control stain with...

01 January 1970 8,661 2 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How to define the sink temperature and film coefficient?

Hi, everyone. I am considering the heat conviction during compacting simulation by Abaqus. I was wondering that how to define the sink temperature and film coefficient? Is there any references...

21 February 2021 6,197 1 View

No title

The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...

18 February 2021 9,538 1 View