No title

PVA/ZnO nanocomposite film

03 December 2018 5,459 2 View

Please let me know why my qpcr is not working properly ?...i m very new in qpcr?

I ran qpcr and got strange results like inverted peak in melt curve, improper amplification curve. I repeated the experiment for optimization but everytime i got same error. Then i performed...

10 June 2018 1,443 3 View

Is there anyone who are working on SHSY5Y cell line? I am looking for a donor who can donate the cell line SHSY5Y.

I will work on Parkinson's disease using SHSY5Y cell line. I have ordered the cell line from NCCS, Pune multiple times. Unfortunately, I have received the cell line contaminated with a slow...

25 July 2017 7,034 2 View

Can i use ampicillin for removing Agrobacterium rhizogenes from the transformed plant ?

Can i use ampicillin sodium salt instead of carbenicillin or cefotaxime for selecting transformed plant? i want to use ampicillin as it is cheaper than other antibiotics.... Thank u

09 May 2017 8,875 10 View

How to work with the cancer patients not accepting their condition?

Most of time they will be a state of denial about stages of cancer,chances of severity, even sometime they are reluctant to discontinue their present job. Sometime It perceived like fear,sometime...

04 May 2016 3,048 2 View

What is the maximum non-toxic concentration of DMSO in Cell culture?

19 April 2016 6,934 2 View

Is there any study explain about determination of sample size of RCTs and qualitative designs?

Think you are going to introduce a innovative therapy and you wanted to do a market survey ,what will be your population size and how to determine this.

10 April 2016 8,468 5 View

Calling a variable from main to another function - any thoughts?

I have a main function which outputs image I, J. I need to read these I, J as inputs in another function (comb.m). I have tried declaring I, J as global but when I run comb.m, it's showing...

11 March 2014 3,775 9 View

Image normalization?

I have done a normalization (0 to 1) of an image. I want to display the normalized image but I am unable to. Is this normalized image similar to binary image?

09 February 2014 341 4 View

Can anyone suggest a feasible application of image enhancement?

I did a project on image enhancement using PSO. I have to continue this for my full semester. What improvement is possible?

02 December 2013 3,618 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View