No title

11 December 2014 2,680 7 View

What could be the reaction products of K2MnO4+HNO3+NaOH+Sucralose=?

10 November 2014 6,884 6 View

Is it possible to substitute chloride with iodide or Iodine-125 in sucralose?

Sucralose is manufactured by the selective chlorination of sucrose (table sugar), which substitutes three of the hydroxyl groups with chlorine. Sucralose (a "chlorinated" carbohydrate) has some...

31 December 2013 7,541 0 View

Can I transfer cell culture from place to place?

05 June 2013 541 11 View

How can I remove urobilin from urine?

I am developing an assay where we need to separate sucralose from urine. The problem is urine (control) gives peak at the same time. I have tried with phase partitioning but it did not work well....

01 February 2013 1,008 4 View

Need protocol for isolation of myenteric plexus from human colon tissue.

I have protocol for smooth muscle cell isolation from human colon. Now I am trying to isolate myenteric plexus from human colon tissue.

31 December 2012 6,095 6 View

How to prepare endotoxin free plasma sample?

I am trying set up some assay where I need to use endotoxin free plasma as control. Do you have any idea?

11 December 2012 7,855 3 View

How can I improve intracellular Calcium measurement in Lymphocytes and smooth muscle cells?

I am trying to measure intracellular calcium in blood lymphocyte and colonic smooth muscle cells. Usually Lymphocytes don't attach on glass coverslip. So I am using poly-l-lysine to enhance...

10 November 2012 2,521 20 View

Is it possible to separate colonic smooth muscle cells from fibroblast using gradient cetrifugation?

I am trying to culture human colonic smooth muscle cells. What I observed in the culture flask is a mixture of cells with tissue debris.

10 November 2012 2,498 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View