Matthias Krienke

4 Questions 46 Answers 0 Followers

Questions related from Matthias Krienke

Is anyone interested in establishing a MoFlo XDP user group?

04 April 2014 2,253 1 View

Cell cycle measurement facs?

I want to do cell cycle mess with facs. Is it better for permeabilization to use 70% methanol or 70% ethanol? How long should I incubate with meth/eth at 4C? What concentration Pi should I use and...

02 February 2014 762 5 View

Working with MAB 263 (GHR)?

I want to look at GH receptor on bovine lymphocytes and HepG2 cells. I only get positive cells with permeabilisation. But I'm wondering, because I'm working with anti-GHR-AK clone MAB263 and I get...

11 November 2013 4,254 0 View

Can I use trypsin (?%) with EDTA (?%) to get a single cell solution from cultured HepG2 cells?

Does anyone have a good protocol to make a single cell solution from cultured liver cells for facs sorting?

09 September 2013 8,721 12 View