Victor Llombart

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Questions related from Victor Llombart

Can anyone suggest us a buffer to detach exosomes from magnetic beads?

We are trying to isolate CD63+ exosomes in order to characterize them by electronic microscopy, Nanosight and ultimately by proteomics/transcriptomics. To isolate them we use the “Total Exosome...

11 November 2016 6,878 6 View

Can someone recommend a low molecular weight ladder for 2-20 Kda protein separation?

We want to separate proteins of 2-20 KDa in tricine gels, stain them with coomassie and cut the bands of interest based on their molecular weight.

05 May 2014 3,733 2 View

Can you suggest me a good antibody for Citrate synthase annalysis by WB?

We want to analyse Citrate synthase in an endothelial cell line and have a reduced sample volume so we cannot lose it performing tests. Could you suggest a good antibody and its best working...

01 January 2014 3,484 2 View

Can anyone suggest a good antibody for Anexin A1 analysis by WB?

We want to perform WB with the secretome of an endothelial cell line, but we have very little sample volume and we cannot spend it testing the antibody. Could you suggest a good antibody with its...

01 January 2014 2,986 3 View

What is the best strategy to validate SILAC results by WB?

In our SILAC experiment we analyzed supernatants from cell cultures. It seems that treated cells secrete more protein than control cells (more or less 3 fold, as checked in many other cultures we...

12 December 2013 7,965 1 View