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11 December 2014 2,680 7 View

What could be the reaction products of K2MnO4+HNO3+NaOH+Sucralose=?

10 November 2014 6,884 6 View

Is it possible to substitute chloride with iodide or Iodine-125 in sucralose?

Sucralose is manufactured by the selective chlorination of sucrose (table sugar), which substitutes three of the hydroxyl groups with chlorine. Sucralose (a "chlorinated" carbohydrate) has some...

31 December 2013 7,541 0 View

Is there any easy and low cost method to check smooth muscle cells viability?

I am working with human colonic smooth muscle cells. The goal is to see drug effects on intracellular calcium level.

11 December 2013 7,751 8 View

Can I transfer cell culture from place to place?

05 June 2013 541 11 View

Need protocol for isolation of myenteric plexus from human colon tissue.

I have protocol for smooth muscle cell isolation from human colon. Now I am trying to isolate myenteric plexus from human colon tissue.

31 December 2012 6,095 6 View

How to prepare endotoxin free plasma sample?

I am trying set up some assay where I need to use endotoxin free plasma as control. Do you have any idea?

11 December 2012 7,855 3 View

How can I improve intracellular Calcium measurement in Lymphocytes and smooth muscle cells?

I am trying to measure intracellular calcium in blood lymphocyte and colonic smooth muscle cells. Usually Lymphocytes don't attach on glass coverslip. So I am using poly-l-lysine to enhance...

10 November 2012 2,521 20 View

Is it possible to separate colonic smooth muscle cells from fibroblast using gradient cetrifugation?

I am trying to culture human colonic smooth muscle cells. What I observed in the culture flask is a mixture of cells with tissue debris.

10 November 2012 2,498 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View