I am trying set up some assay where I need to use endotoxin free plasma as control. Do you have any idea?
Plasma is essentially endotoxin free by itself. If you used pyrogen free labware to collect the plasma, you should be OK.
Plasma is essentially free of active (unbound) endotoxin. 99.99% of endotoxin in normal Plasma is inactive (bound).
If necessary, levels of endotoxins can be measured using the limulus amebocyte lysate (LAL) assay, which is the mainstay for its detection. There are also some commercially available columns which make it possible to remove endotoxins from a solution, and presumably from plasma as well (but I have no experiences with these). Alternatively, if your experiments involve cells that might react to endotoxins, it is possible to use polymyxin B, which is an inhibitor of endotoxin detection. As mentionned above, it is of course essential to make sure that there is no contamination by endotoxins while handling plasma samples, and using pyrogen-free labware is definitely important for this purpose.
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